Antioxidant Activity of Rosmarinic Acid Extracted and Purified from .
Journal:
Archives of Razi Institute
PMID:
35355734
Abstract
Rosmarinic acid was obtained from methanolic extract of under a reflux condenser. The current study aimed to evaluate the in vitro antioxidant activities and rosmarinic acid levels of the methanol extracts of . The analysis of the sample by high-performance liquid chromatography technique (HPLC) indicated that rosmarinic acid was present in high concentration 1.9 mg/mL in the extract. Purification was carried out by column chromatography to give 0.020 g from 1 g of crude extract, and then the antioxidant activity of purified rosmarinic acid was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), H2O2 scavenging, and REDOX methods. It was revealed that the anti-oxidant potential of the rosmarinic acid extract was greater than 95% (at 100 µg/mL) for DPPH assay and 87.83% (at 100 µg/ml) for H2O2 scavenging assay. This study was performed by using a reflux methanolic extraction of . This possible instructional technique proved to be a quick and successful method for retaining the antioxidant properties of rosmarinic acid. The rosmarinic acid content was determined using HPLC.