Deep learning-enabled analysis reveals distinct neuronal phenotypes induced by aging and cold-shock.

Journal: BMC biology
Published Date:

Abstract

BACKGROUND: Access to quantitative information is crucial to obtain a deeper understanding of biological systems. In addition to being low-throughput, traditional image-based analysis is mostly limited to error-prone qualitative or semi-quantitative assessment of phenotypes, particularly for complex subcellular morphologies. The PVD neuron in Caenorhabditis elegans, which is responsible for harsh touch and thermosensation, undergoes structural degeneration as nematodes age characterized by the appearance of dendritic protrusions. Analysis of these neurodegenerative patterns is labor-intensive and limited to qualitative assessment.

Authors

  • Sahand Saberi-Bosari
    Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USA.
  • Kevin B Flores
    Department of Mathematics, North Carolina State University, Raleigh, NC, 27695, USA.
  • Adriana San-Miguel
    Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USA. asanmiguel@ncsu.edu.