Unlocking the efficiency of genomics laboratories with robotic liquid-handling.

Journal: BMC genomics
Published Date:

Abstract

In research and clinical genomics laboratories today, sample preparation is the bottleneck of experiments, particularly when it comes to high-throughput next generation sequencing (NGS). More genomics laboratories are now considering liquid-handling automation to make the sequencing workflow more efficient and cost effective. The question remains as to its suitability and return on investment. A number of points need to be carefully considered before introducing robots into biological laboratories. Here, we describe the state-of-the-art technology of both sophisticated and do-it-yourself (DIY) robotic liquid-handlers and provide a practical review of the motivation, implications and requirements of laboratory automation for genome sequencing experiments.

Authors

  • Houriiyah Tegally
    Kwazulu-Natal Research and Innovation Sequencing Platform (KRISP), College of Health Sciences, K-RITH Tower Building, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, 719 Umbilo Road, Durban, South Africa. houriiyah.tegally@gmail.com.
  • James Emmanuel San
    Kwazulu-Natal Research and Innovation Sequencing Platform (KRISP), College of Health Sciences, K-RITH Tower Building, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, 719 Umbilo Road, Durban, South Africa.
  • Jennifer Giandhari
    Kwazulu-Natal Research and Innovation Sequencing Platform (KRISP), College of Health Sciences, K-RITH Tower Building, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, 719 Umbilo Road, Durban, South Africa.
  • Tulio de Oliveira
    Kwazulu-Natal Research and Innovation Sequencing Platform (KRISP), College of Health Sciences, K-RITH Tower Building, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, 719 Umbilo Road, Durban, South Africa. deoliveira@ukzn.ac.za.