Incubation with Cholesterol-loaded Cyclodextrin and Subsequent Dilution in Partially Deoxygenated Extender Improves the Freezability of Cross-bred Bull Sperm.

Journal: Cryo letters
PMID: 33988663

Abstract

BACKGROUND: The cryopreservation process induces osmotic stress, membrane changes and production of reactive oxygen species resulting in damage to the spermatozoa. Together, the presence of oxygen in the extender aggravates the oxidative stress that further reduces the cryosurvival rate of sperm cells.

Authors

  • R Katiyar
    Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India. rahul.katiyarvet@gmail.com.
  • S K Ghosh
    Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
  • J K Prasad
    Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
  • A Kumar
    Department of Chemical Engineering, College of Engineering, Qatar University Doha 2713 Qatar.
  • L Bhutia
    Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
  • V Gupta
    Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
  • R Rautela
    Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.

Keywords

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