A multi-conformational virtual screening approach based on machine learning targeting PI3Kγ.

Journal: Molecular diversity
PMID:

Abstract

Nowadays, more and more attention has been attracted to develop selective PI3Kγ inhibitors, but the unique structural features of PI3Kγ protein make it a very big challenge. In the present study, a virtual screening strategy based on machine learning with multiple PI3Kγ protein structures was developed to screen novel PI3Kγ inhibitors. First, six mainstream docking programs were chosen to evaluate their scoring power and screening power; CDOCKER and Glide show satisfactory reliability and accuracy against the PI3Kγ system. Next, virtual screening integrating multiple PI3Kγ protein structures was demonstrated to significantly improve the screening enrichment rate comparing to that with an individual protein structure. Last, a multi-conformational Naïve Bayesian Classification model with the optimal docking programs was constructed, and it performed a true capability in the screening of PI3Kγ inhibitors. Taken together, the current study could provide some guidance for the docking-based virtual screening to discover novel PI3Kγ inhibitors.

Authors

  • Jingyu Zhu
    School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, Jiangsu, China. jingyuzhu@jiangnan.edu.cn.
  • Yingmin Jiang
    School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, Jiangsu, China.
  • Lei Jia
    Department of AIDS Research, State Key Laboratory of Pathogen and Biosafety, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
  • Lei Xu
    Key Laboratory of Biomedical Information Engineering of the Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, China.
  • Yanfei Cai
    School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, Jiangsu, China.
  • Yun Chen
  • Nannan Zhu
    School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, Jiangsu, China.
  • Huazhong Li
    School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, China.
  • Jian Jin
    Department of Agricultural and Biological Engineering, Purdue University, 225 S. University St., West Lafayette, IN 47907, USA.