Deep-learning on-chip light-sheet microscopy enabling video-rate volumetric imaging of dynamic biological specimens.
Journal:
Lab on a chip
Published Date:
Sep 14, 2021
Abstract
Volumetric imaging of dynamic signals in a large, moving, and light-scattering specimen is extremely challenging, owing to the requirement on high spatiotemporal resolution and difficulty in obtaining high-contrast signals. Here we report that through combining a microfluidic chip-enabled digital scanning light-sheet illumination strategy with deep-learning based image restoration, we can realize isotropic 3D imaging of a whole crawling larva on an ordinary inverted microscope at a single-cell resolution and a high volumetric imaging rate up to 20 Hz. Enabled with high performances even unmet by current standard light-sheet fluorescence microscopes, we record the neural activities during the forward and backward crawling of a 1st instar larva, and successfully correlate the calcium spiking of motor neurons with the locomotion patterns.