Spectroscopy and imaging technologies coupled with machine learning for the assessment of the microbiological spoilage associated to ready-to-eat leafy vegetables.

Journal: International journal of food microbiology
Published Date:

Abstract

Based on both new and previously utilized experimental data, the present study provides a comparative assessment of sensors and machine learning approaches for evaluating the microbiological spoilage of ready-to-eat leafy vegetables (baby spinach and rocket). Fourier-transform infrared (FTIR), near-infrared (NIR), visible (VIS) spectroscopy and multispectral imaging (MSI) were used. Two data partitioning approaches and two algorithms, namely partial least squares regression and support vector regression (SVR), were evaluated. Concerning baby spinach, when model testing was performed on samples randomly selected, the performance was better than or similar to the one attained when testing was performed based on dynamic temperatures data, depending on the applied analytical technology. The two applied algorithms yielded similar model performances for the majority of baby spinach cases. Regarding rocket, the random data partitioning approach performed considerably better results in almost all cases of sensor/algorithm combination. Furthermore, SVR algorithm resulted in considerably or slightly better model performances for the FTIR, VIS and NIR sensors, depending on the data partitioning approach. However, PLSR algorithm provided better models for the MSI sensor. Overall, the microbiological spoilage of baby spinach was better assessed by models derived mainly from the VIS sensor, while FTIR and MSI were more suitable in rocket. According to the findings of this study, a distinct sensor and computational analysis application is needed for each vegetable type, suggesting that there is not a single combination of analytical approach/algorithm that could be applied successfully in all food products and throughout the food supply chain.

Authors

  • Evanthia Manthou
    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food & Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
  • Apostolos Karnavas
    School of Food and Nutritional Sciences, Department of Food Science and Human Nutrition, Laboratory of Microbiology and Biotechnology of Foods, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece.
  • Lemonia-Christina Fengou
    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food & Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
  • Anastasia Bakali
    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food & Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
  • Alexandra Lianou
    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food & Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece; Division of Genetics, Cell Biology and Development, Department of Biology, University of Patras, 26504 Patras, Greece.
  • Panagiotis Tsakanikas
    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece.
  • George-John E Nychas
    Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food & Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece. Electronic address: gjn@aua.gr.