Raman spectroscopy-based adversarial network combined with SVM for detection of foodborne pathogenic bacteria.

Journal: Talanta
Published Date:

Abstract

Raman spectroscopy combined with artificial intelligence algorithms have been widely explored and focused on in recent years for food safety testing. It is still a challenge to overcome the cumbersome culture process of bacteria and the need for a large number of samples, which hinder qualitative analysis, to obtain a high classification accuracy. In this paper, we propose a method based on Raman spectroscopy combined with generative adversarial network and multiclass support vector machine to classify foodborne pathogenic bacteria. 30,000 iterations of generative adversarial network are trained for three strains of bacteria, generative model G generates data similar to the actual samples, discriminant model D verifies the accuracy of the generated data, and 19 feature variables are obtained by selecting the feature bands according to the Raman spectroscopy pattern. Better classification results are obtained by optimising the parameters of the multi-class support vector machine, etc. Our detection and classification method not only solves the problem of needing a large number of samples as training set, but also improves the accuracy of the classification model. Therefore, this GAN-SVM classification model provides a new idea for the detection of bacteria based on Raman spectroscopy technology combined with artificial intelligence algorithms.

Authors

  • Yuwan Du
    Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environment and Operational Medicine, Tianjin, 300050, PR China.
  • Dianpeng Han
    Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environment and Operational Medicine, Tianjin, 300050, PR China.
  • Sha Liu
    School of Public Health, Binzhou Medical University, Yantai, China.
  • Xuan Sun
    State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China.
  • Baoan Ning
    Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, PR China.
  • Tie Han
    Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environment and Operational Medicine, Tianjin, 300050, PR China.
  • Jiang Wang
    School of Electrical Engineering and Automation, Tianjin University, Tianjin, 300072, People's Republic of China.
  • Zhixian Gao
    Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, PR China. Electronic address: gaozhx@163.com.