Comparison of deep learning models for digital H&E staining from unpaired label-free multispectral microscopy images.

Journal: Computer methods and programs in biomedicine
Published Date:

Abstract

BACKGROUND AND OBJECTIVE: This paper presents the quantitative comparison of three generative models of digital staining, also known as virtual staining, in H&E modality (i.e., Hematoxylin and Eosin) that are applied to 5 types of breast tissue. Moreover, a qualitative evaluation of the results achieved with the best model was carried out. This process is based on images of samples without staining captured by a multispectral microscope with previous dimensional reduction to three channels in the RGB range.

Authors

  • Jesus Salido
    IEEAC Dept. (ESI-UCLM), P de la Universidad 4, Ciudad Real, 13071, Spain. Electronic address: jesus.salido@uclm.es.
  • Noelia Vállez
    VISILAB Group, ETSI Industriales, University of Castilla-La Mancha, Ciudad Real, Spain.
  • Lucía González-López
    Hospital Gral. Universitario de C.Real (HGUCR), C. Obispo Rafael Torija s/n, Ciudad Real, 13005, Spain.
  • Oscar Deniz
    VISILAB Group, ETSI Industriales, University of Castilla-La Mancha, Ciudad Real, Spain.
  • Gloria Bueno
    VISILAB Group, ETSI Industriales, University of Castilla-La Mancha, Ciudad Real, Spain.