AIMC Topic: Staining and Labeling

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Proximity Effects Leveraged in Ligand-Directed Chemical Labeling of Natural Proteins under Live Conditions.

Accounts of chemical research
ConspectusCovalent chemical labeling of proteins is central to chemical biology, offering functional modifications beyond imaging probes. While genetically engineered systems using self-labeling tags (e.g., HaloTag, SNAP-Tag) or genetic code expansio...

Mobile phone-based plasmodium parasites stage detection from Giemsa stained blood smear by convolutional neural networks.

Parasitology research
Plasmodium vivax is a malaria parasite with a broad geographic distribution worldwide. The unique biological characteristics of P. vivax, such as early gametocytogenesis and its latent hypnozoite stage, make it more difficult to control compared to P...

FLASH: innovative integrated enzymatic-fluorescent labeling for automated muscle fiber typing, metabolic and morphometric analysis.

Skeletal muscle
BACKGROUND: Skeletal muscle is a dynamic tissue capable of structural and metabolic remodeling in response to physiological and pathological stimuli. These adaptations are central to understanding the mechanisms underlying conditions such as genetic ...

Automated quantification of Ki-67 expression in breast cancer from H&E-stained slides using a transformer-based regression model.

Breast cancer research : BCR
BACKGROUND: Accurate quantification of the Ki-67 proliferation index is essential for breast cancer prognosis and treatment planning. Current automated methods, including classical and deep learning approaches based on cell detection or segmentation,...

Assessing the risk of recurrence in early-stage breast cancer through H&E stained whole slide images.

Scientific reports
Accurate prediction of the likelihood of recurrence is important in the selection of postoperative treatment for patients with early-stage breast cancer. In this study, we investigated whether deep learning algorithms can predict patients' risk of re...

Nondisruptive inducible labeling of ER-membrane contact sites using the Lamin B receptor.

PLoS biology
Membrane contact sites (MCSs) are areas of close proximity between organelles that allow the exchange of material, among other roles. The endoplasmic reticulum (ER) has MCSs with a variety of organelles in the cell. MCSs are dynamic, responding to ch...

Multivariate and Machine Learning-Derived Virtual Staining and Biochemical Quantification of Cancer Cells through Raman Hyperspectral Imaging.

Analytical chemistry
Advances in virtual staining and spatial omics have revolutionized our ability to explore cellular architecture and molecular composition with unprecedented detail. Virtual staining techniques, which rely on computational algorithms to map molecular ...

Pixel super-resolved virtual staining of label-free tissue using diffusion models.

Nature communications
Virtual staining of tissue offers a powerful tool for transforming label-free microscopy images of unstained tissue into equivalents of histochemically stained samples. This study presents a diffusion model-based pixel super-resolution virtual staini...

A Benchmark for Virus Infection Reporter Virtual Staining in Fluorescence and Brightfield Microscopy.

Scientific data
Detecting virus-infected cells in light microscopy requires a reporter signal commonly achieved by immunohistochemistry or genetic engineering. While classification-based machine learning approaches to the detection of virus-infected cells have been ...

Revealing 3D microanatomical structures of unlabeled thick cancer tissues using holotomography and virtual H&E staining.

Nature communications
In histopathology, acquiring subcellular-level three-dimensional (3D) tissue structures efficiently and without damaging the tissues during serial sectioning and staining remains a formidable challenge. We address this by integrating holotomography w...