AI Medical Compendium Topic

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Staining and Labeling

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CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms.

Frontiers in cellular and infection microbiology
INTRODUCTION: Accurately assessing biofilm viability is essential for evaluating both biofilm formation and the efficacy of antibacterial treatments. Traditional SYTO9 and propidium iodide (PI) live/dead staining in biofilm viability assays often ace...

High-rate emphasized DeepLabV3Plus for Semantic Segmentation of Breast Cancer-related Hematoxylin and Eosin-stained Images.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Deep learning algorithms have been successfully adopted to extract meaningful information from digital images, yet many of them have been untapped in the semantic image segmentation of histopathology images. In this paper, we propose a deep convoluti...

Learnable color space conversion and fusion for stain normalization in pathology images.

Medical image analysis
Variations in hue and contrast are common in H&E-stained pathology images due to differences in slide preparation across various institutions. Such stain variations, while not affecting pathologists much in diagnosing the biopsy, pose significant cha...

Multiple instance learning-based prediction of programmed death-ligand 1 (PD-L1) expression from hematoxylin and eosin (H&E)-stained histopathological images in breast cancer.

PeerJ
Programmed death-ligand 1 (PD-L1) is an important biomarker increasingly used as a predictive marker in breast cancer immunotherapy. Immunohistochemical quantification remains the standard method for assessment. However, it presents challenges relate...

Spatial transcriptome reveals histology-correlated immune signature learnt by deep learning attention mechanism on H&E-stained images for ovarian cancer prognosis.

Journal of translational medicine
BACKGROUND: The ability to predict the prognosis of patients with ovarian cancer can greatly improve disease management. However, the knowledge on the mechanism of the prediction is limited. We sought to deconvolute the attention feature learnt by a ...

Virtual Gram staining of label-free bacteria using dark-field microscopy and deep learning.

Science advances
Gram staining has been a frequently used staining protocol in microbiology. It is vulnerable to staining artifacts due to, e.g., operator errors and chemical variations. Here, we introduce virtual Gram staining of label-free bacteria using a trained ...

Deeply supervised two stage generative adversarial network for stain normalization.

Scientific reports
The color variations present in histopathological images pose a significant challenge to computational pathology and, consequently, negatively affect the performance of certain pathological image analysis methods, especially those based on deep learn...

Artificial intelligence-based virtual staining platform for identifying tumor-associated macrophages from hematoxylin and eosin-stained images.

European journal of cancer (Oxford, England : 1990)
BACKGROUND: Virtual staining is an artificial intelligence-based approach that transforms pathology images between stain types, such as hematoxylin and eosin (H&E) to immunohistochemistry (IHC), providing a tissue-preserving and efficient alternative...

Toward Accurate Deep Learning-Based Prediction of Ki67, ER, PR, and HER2 Status From H&E-Stained Breast Cancer Images.

Applied immunohistochemistry & molecular morphology : AIMM
Despite improvements in machine learning algorithms applied to digital pathology, only moderate accuracy, to predict molecular information from histology alone, has been achieved so far. One of the obstacles is the lack of large data sets to properly...

Machine learning prediction of HER2-low expression in breast cancers based on hematoxylin-eosin-stained slides.

Breast cancer research : BCR
BACKGROUND: Treatment with HER2-targeted therapies is recommended for HER2-positive breast cancer patients with HER2 gene amplification or protein overexpression. Interestingly, recent clinical trials of novel HER2-targeted therapies demonstrated pro...