Digital Microscopy Augmented by Artificial Intelligence to Interpret Bone Marrow Samples for Hematological Diseases.

Journal: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
PMID:

Abstract

Analysis of bone marrow aspirates (BMAs) is an essential step in the diagnosis of hematological disorders. This analysis is usually performed based on a visual examination of samples under a conventional optical microscope, which involves a labor-intensive process, limited by clinical experience and subject to high observer variability. In this work, we present a comprehensive digital microscopy system that enables BMA analysis for cell type counting and differentiation in an efficient and objective manner. This system not only provides an accessible and simple method to digitize, store, and analyze BMA samples remotely but is also supported by an Artificial Intelligence (AI) pipeline that accelerates the differential cell counting process and reduces interobserver variability. It has been designed to integrate AI algorithms with the daily clinical routine and can be used in any regular hospital workflow.

Authors

  • David Bermejo-Pelaez
  • Sandra Rueda Charro
    Spotlab, P.º de Juan XXIII, 36B, Madrid 28040, Spain.
  • María García Roa
    Department of Hematology, Hospital Universitario Fundación Alcorcón, C. Budapest, 1, Alcorcón 28922, Madrid, Spain.
  • Roberto Trelles-Martínez
    Department of Hematology, Hospital Universitario Fundación Alcorcón, C. Budapest, 1, Alcorcón 28922, Madrid, Spain.
  • Alejandro Bobes-Fernández
    Department of Hematology, Hospital Universitario Fundación Alcorcón, C. Budapest, 1, Alcorcón 28922, Madrid, Spain.
  • Marta Hidalgo Soto
    Vall Hebron Institute of Oncology (VHIO), Carrer de Natzaret, 115-117, Horta-Guinardó, Barcelona 08035, Spain.
  • Roberto García-Vicente
    Department of Translational Hematology, Research Institute Hospital 12 de Octubre (imas12), Av. de Córdoba, s/n, Madrid 28041, Spain.
  • María Luz Morales
    Department of Translational Hematology, Research Institute Hospital 12 de Octubre (imas12), Av. de Córdoba, s/n, Madrid 28041, Spain.
  • Alba Rodríguez-García
    Department of Translational Hematology, Research Institute Hospital 12 de Octubre (imas12), Av. de Córdoba, s/n, Madrid 28041, Spain.
  • Alejandra Ortiz-Ruiz
    Department of Translational Hematology, Research Institute Hospital 12 de Octubre (imas12), Av. de Córdoba, s/n, Madrid 28041, Spain.
  • Alberto Blanco Sánchez
    Department of Hematology, Hospital Universitario 12 de Octubre, Av. de Córdoba, s/n, Madrid 28041, Spain.
  • Adriana Mousa Urbina
    Spotlab, P.º de Juan XXIII, 36B, Madrid 28040, Spain.
  • Elisa Álamo
    Spotlab, Madrid, Spain.
  • Lin Lin
    Central Laboratory, The First Affiliated Hospital of Xiamen University, Xiamen, China, zhibinli33@163.com, liusuhuan@xmu.edu.cn.
  • Elena Dacal
    Spotlab, Madrid, Spain.
  • Daniel Cuadrado
    Spotlab, Madrid, Spain.
  • María Postigo
    Spotlab, Madrid, Spain.
  • Alexander Vladimirov
    Spotlab, Madrid, Spain.
  • Jaime Garcia-Villena
    Spotlab, P.º de Juan XXIII, 36B, Madrid 28040, Spain.
  • Andrés Santos
    Biomedical Image Technologies Laboratory, ETSI Telecomunicación, Universidad Politécnica de Madrid, Av. Complutense, 30, Madrid 28040, Spain.
  • Maria Jesus Ledesma-Carbayo
  • Rosa Ayala
    Department of Translational Hematology, Research Institute Hospital 12 de Octubre (imas12), Av. de Córdoba, s/n, Madrid 28041, Spain.
  • Joaquín Martínez-López
    Department of Translational Hematology, Research Institute Hospital 12 de Octubre (imas12), Av. de Córdoba, s/n, Madrid 28041, Spain.
  • María Linares
    Department of Translational Hematology, Research Institute Hospital 12 de Octubre (imas12), Av. de Córdoba, s/n, Madrid 28041, Spain.
  • Miguel Luengo-Oroz
    Spotlab, Madrid, Spain.