MiRNATIP: a SOM-based miRNA-target interactions predictor.

Journal: BMC bioinformatics
Published Date:

Abstract

BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNA sequences with regulatory functions to post-transcriptional level for several biological processes, such as cell disease progression and metastasis. MiRNAs interact with target messenger RNA (mRNA) genes by base pairing. Experimental identification of miRNA target is one of the major challenges in cancer biology because miRNAs can act as tumour suppressors or oncogenes by targeting different type of targets. The use of machine learning methods for the prediction of the target genes is considered a valid support to investigate miRNA functions and to guide related wet-lab experiments. In this paper we propose the miRNA Target Interaction Predictor (miRNATIP) algorithm, a Self-Organizing Map (SOM) based method for the miRNA target prediction. SOM is trained with the seed region of the miRNA sequences and then the mRNA sequences are projected into the SOM lattice in order to find putative interactions with miRNAs. These interactions will be filtered considering the remaining part of the miRNA sequences and estimating the free-energy necessary for duplex stability.

Authors

  • Antonino Fiannaca
    National Research Council of Italy, ICAR-CNR, via Ugo La Malfa 153, Palermo, 90146, Italy. fiannaca@pa.icar.cnr.it.
  • Massimo La Rosa
    National Research Council of Italy, ICAR-CNR, via Ugo La Malfa 153, Palermo, 90146, Italy.
  • Laura La Paglia
    National Research Council of Italy, ICAR-CNR, via Ugo La Malfa 153, Palermo, 90146, Italy.
  • Riccardo Rizzo
    National Research Council of Italy, ICAR-CNR, via Ugo La Malfa 153, Palermo, 90146, Italy.
  • Alfonso Urso
    Istituto di Calcolo e Reti ad Alte Prestazioni-Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy.