Food research international (Ottawa, Ont.)
32156354
A new Ultrasound-Ultrafiltration-Assisted Alkaline Isoelectric Precipitation (UUAAIP) technique was employed for producing alfalfa protein isolate from alfalfa leaves. A five-factor-three-level Box-Behnken Design (BBD) was used to optimize the UUAAIP...
Isoelectric focusing (IEF) is a powerful tool for resolving complex protein samples, which generates IEF patterns consisting of multiplex analyte bands. However, the interpretation of IEF patterns requires the careful selection of isoelectric point (...