New Derivatives of Pyridoxine Exhibit High Antibacterial Activity against Biofilm-Embedded Staphylococcus Cells.

Journal: BioMed research international
PMID: 26839888

Abstract

Opportunistic bacteria Staphylococcus aureus and Staphylococcus epidermidis often form rigid biofilms on tissues and inorganic surfaces. In the biofilm bacterial cells are embedded in a self-produced polysaccharide matrix and thereby are inaccessible to biocides, antibiotics, or host immune system. Here we show the antibacterial activity of newly synthesized cationic biocides, the quaternary ammonium, and bisphosphonium salts of pyridoxine (vitamin B6) against biofilm-embedded Staphylococci. The derivatives of 6-hydroxymethylpyridoxine were ineffective against biofilm-embedded S. aureus and S. epidermidis at concentrations up to 64 μg/mL, although all compounds tested exhibited low MICs (2 μg/mL) against planktonic cells. In contrast, the quaternary ammonium salt of pyridoxine (N,N-dimethyl-N-((2,2,8-trimethyl-4H-[1,3]dioxino[4,5-c]pyridin-5-yl)methyl)octadecan-1-aminium chloride (3)) demonstrated high biocidal activity against both planktonic and biofilm-embedded bacteria. Thus, the complete death of biofilm-embedded S. aureus and S. epidermidis cells was obtained at concentrations of 64 and 16 μg/mL, respectively. We suggest that the quaternary ammonium salts of pyridoxine are perspective to design new synthetic antibiotics and disinfectants for external application against biofilm-embedded cells.

Authors

  • Airat R Kayumov
    Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.
  • Aliya A Nureeva
    Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.
  • Elena Yu Trizna
    Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.
  • Guzel R Gazizova
    Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.
  • Mikhail I Bogachev
    Biomedical Engineering Research Center, Saint Petersburg Electrotechnical University, Professor Popov Street 5, Saint Petersburg 197376, Russia.
  • Nikita V Shtyrlin
    Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.
  • Mikhail V Pugachev
    Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.
  • Sergey V Sapozhnikov
    Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.
  • Yurii G Shtyrlin
    Kazan Federal University, Kremlevskaya Street 18, Kazan 420008, Russia.

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