Mutagenic Potential ofBos taurus Papillomavirus Type 1 E6 Recombinant Protein: First Description.

Journal: BioMed research international
PMID: 26783529

Abstract

Bovine papillomavirus (BPV) is considered a useful model to study HPV oncogenic process. BPV interacts with the host chromatin, resulting in DNA damage, which is attributed to E5, E6, and E7 viral oncoproteins activity. However, the oncogenic mechanisms of BPV E6 oncoprotein per se remain unknown. This study aimed to evaluate the mutagenic potential of Bos taurus papillomavirus type 1 (BPV-1) E6 recombinant oncoprotein by the cytokinesis-block micronucleus assay (CBMNA) and comet assay (CA). Peripheral blood samples of five calves were collected. Samples were subjected to molecular diagnosis, which did not reveal presence of BPV sequences. Samples were treated with 1 μg/mL of BPV-1 E6 oncoprotein and 50 μg/mL of cyclophosphamide (positive control). Negative controls were not submitted to any treatment. The samples were submitted to the CBMNA and CA. The results showed that BPV E6 oncoprotein induces clastogenesis per se, which is indicative of genomic instability. These results allowed better understanding the mechanism of cancer promotion associated with the BPV E6 oncoprotein and revealed that this oncoprotein can induce carcinogenesis per se. E6 recombinant oncoprotein has been suggested as a possible vaccine candidate. Results pointed out that BPV E6 recombinant oncoprotein modifications are required to use it as vaccine.

Authors

  • Rodrigo Pinheiro Araldi
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil; Programa de Pós-Graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), 05508-900 São Paulo, SP, Brazil.
  • Jacqueline Mazzuchelli-de-Souza
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil; Programa de Pós-Graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), 05508-900 São Paulo, SP, Brazil.
  • Diego Grando Modolo
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Edislane Barreiros de Souza
    Laboratório de Biologia Molecular, Genética e Mutagênese, Departamento de Biologia, Faculdade de Ciências e Letras de Assis (FCLA), Universidade Estadual Paulista "Júlio de Mesquista Filho" (UNESP), 19806-900 Assis, SP, Brazil.
  • Thatiana Corrêa de Melo
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Diva Denelle Spadacci-Morena
    Laboratório de Fisiopatologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Roberta Fiusa Magnelli
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Márcio Augusto Caldas Rocha de Carvalho
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Paulo Luis de Sá Júnior
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Rodrigo Franco de Carvalho
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Willy Beçak
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.
  • Rita de Cassia Stocco
    Laboratório de Genética, Instituto Butantan, 05503-900 São Paulo, SP, Brazil.

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