CPHNet: a novel pipeline for anti-HAPE drug screening via deep learning-based Cell Painting scoring.

Journal: Respiratory research
PMID: 40057746

Abstract

BACKGROUND: High altitude pulmonary edema (HAPE) poses a significant medical challenge to individuals ascending rapidly to high altitudes. Hypoxia-induced cellular morphological changes in the alveolar-capillary barrier such as mitochondrial structural alterations and cytoskeletal reorganization, play a crucial role in the pathogenesis of HAPE. These morphological changes are critical in understanding the cellular response to hypoxia and represent potential therapeutic targets. However, there is still a lack of effective and valid drug discovery strategies for anti-HAPE treatments based on these cellular morphological features. This study aims to develop a pipeline that focuses on morphological alterations in Cell Painting images to identify potential therapeutic agents for HAPE interventions.

Authors

  • De-Zhi Sun
    Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, No. 27, Taiping Road, Haidian District, Beijing, 100850, China.
  • Xi-Ru Yang
    Department of Pharmacy, Medical College of Qinghai University, Xining, Qinghai, 810001, China.
  • Cong-Shu Huang
    Academy of Military Medical Sciences PLA Academy of Military Sciences, Beijing 100039, China.
  • Zhi-Jie Bai
    Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, No. 27, Taiping Road, Haidian District, Beijing, 100850, China.
  • Pan Shen
    Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, China.
  • Zhe-Xin Ni
    Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, No. 27, Taiping Road, Haidian District, Beijing, 100850, China.
  • Chao-Ji Huang-Fu
    Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, No. 27, Taiping Road, Haidian District, Beijing, 100850, China.
  • Yang-Yi Hu
    Department of Pharmacy, Medical College of Qinghai University, Xining, Qinghai, 810001, China.
  • Ning-Ning Wang
    Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, Hunan, P.R. China.
  • Xiang-Lin Tang
    Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, No. 27, Taiping Road, Haidian District, Beijing, 100850, China.
  • Yong-Fang Li
    Department of Pharmacy, Medical College of Qinghai University, Xining, Qinghai, 810001, China.
  • Yue Gao
    Institute of Medical Technology, Peking University Health Science Center, Beijing, China.
  • Wei Zhou
    Department of Eye Function Laboratory, Eye Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

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