Volatile constituents and in vitro activity of Syzygium aromaticum flower buds (clove) against human cancer cell lines.

Journal: Pakistan journal of pharmaceutical sciences
PMID: 33867344

Abstract

The methanolic extract (SA-EXT) of Syzygium aromaticum flower buds and its fractions tested against three human cancer cell lines viz uterine cervix (HeLa), breast (MCF-7) and lung NCI (H-460) using sulforhodamine-B assay. The ethyl acetate soluble sub fraction (SA-EAS) was active only against HeLa cells with GIvalue of 36± 3.4μg/mL. The most active sub-fraction (SA-PES-Fr-2) showed growth inhibition (GI: 36, 50 and 68μg/ml against MCF-7, HeLa and NCI-H-460 cancer cell lines, respectively) with cytotoxic effect LC= 88 ± 3.4 μg/mL against HeLa and LC=86 ± 2.8 μg/mL against MCF-7. The most active sub-fraction (SA-PES-Fr-2) analyzed by GC and GC-MS techniques revealed that eugenol was most abundant (85.34%) along with minor constituents. Thus it can be concluded that growth inhibitory and cytotoxic effect residing in Syzygium aromaticum flower buds (clove) is more likely due to eugenol.

Authors

  • - Sara
    HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan/Department of Chemistry University of Karachi, Karachi, Pakistan.
  • Sabira Begum
    H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
  • Syed Nawazish Ali
    Department of Chemistry University of Karachi, Karachi, Pakistan.
  • Ahsana Dar Farooq
    Hamdard Al-Majeed College of Eastern Medicine Hamdard University, Karachi, Pakistan.
  • Faheema Siddiqui
    Faculty of Pharmaceutical Sciences, Dow College of Pharmacy, Dow University of Health Sciences, Karachi, Pakistan.
  • Bina S Siddiqui
    H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan; Pakistan Academy of Sciences, 3-Constitution Avenue, G-5/2, Islamabad, Pakistan. Electronic address: siddiqui_bina@yahoo.com.
  • Anjum Ayub
    HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan/ NED University of Engineering & Technology, Karachi, Pakistan.
  • Mashhad Fatima
    HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan.

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