Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses.

Journal: Frontiers in cellular and infection microbiology
PMID: 39935536

Abstract

INTRODUCTION: Viral calf diarrhea poses a significant challenge to the cattle industry worldwide due to its high morbidity and mortality rates, leading to substantial economic losses. The clinical symptoms associated with various diarrhea pathogens often overlap, complicating accurate diagnosis; thus, there is an urgent need for rapid and precise diagnostic methods to improve prevention and treatment efforts. In this study, we developed a one-step multiplex reverse-transcription quantitative real-time polymerase chain reaction (mRT-qPCR) that enables the simultaneous detection of three key viral pathogens responsible for calf diarrhea: bovine kobuvirus (BKoV), bovine astrovirus (BoAstV), and bovine torovirus (BToV). However, development of accurate and rapid methods to distinguish these three viruses is helpful for the early detection, disease surveillance, and control of viral calf diarrhea.

Authors

  • Dequan Yang
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Li Ma
    Department of Technological Research and Development, Hunan Guanmu Biotech Co., Ltd, Changsha, China.
  • Zhongping Yang
    Department of Technological Research and Development, Hunan Guanmu Biotech Co., Ltd, Changsha, China.
  • Xianchao Yang
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Jian Wang
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Houbin Ju
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Chunguang Lu
    Department of Veterinary Laboratory, Jinshan District Animal Disease Control Center, Shanghai, China.
  • Yonggang Weng
    Department of Veterinary Laboratory, Jinshan District Animal Disease Control Center, Shanghai, China.
  • Heping Zhao
    Department of Technological Research and Development, Hunan Guanmu Biotech Co., Ltd, Changsha, China.
  • Haixiao Shen
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Xin Li
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Feifei Ge
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Xiaoxu Wang
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Xiujuan Wu
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Meng Xiang
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Guidan Feng
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Congsheng Tang
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Shixin Huang
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.
  • Hongjin Zhao
    Veterinary Diagnostic Center, Shanghai Animal Disease Control Center, Shanghai, China.

Keywords

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