AIMC Topic: DNA Primers

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AutoPVPrimer: A comprehensive AI-Enhanced pipeline for efficient plant virus primer design and assessment.

PloS one Jan 30, 2025
Plant viruses pose a significant threat to global agriculture and require efficient tools for their timely detection. We present AutoPVPrimer, an innovative pipeline that integrates artificial intelligence (AI) and machine learning to accelerate the ...
Software DNA Primers Plant Viruses Artificial Intelligence Machine Learning
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Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses.

Frontiers in cellular and infection microbiology Jan 28, 2025
INTRODUCTION: Viral calf diarrhea poses a significant challenge to the cattle industry worldwide due to its high morbidity and mortality rates, leading to substantial economic losses. The clinical symptoms associated with various diarrhea pathogens o...
Torovirus Multiplex Polymerase Chain Reaction DNA Primers Diarrhea Animals Reproducibility of Results Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Cattle Diseases Diarrhea Viruses, Bovine Viral Cattle Sensitivity and Specificity Kobuvirus Bovine Virus Diarrhea-Mucosal Disease Feces
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Prediction of PCR amplification from primer and template sequences using recurrent neural network.

Scientific reports Apr 5, 2021
We have developed a novel method to predict the success of PCR amplification for a specific primer set and DNA template based on the relationship between the primer sequence and the template. To perform the prediction using a recurrent neural network...
Base Sequence Polymerase Chain Reaction Templates, Genetic DNA Primers Computational Biology Algorithms Reproducibility of Results Neural Networks, Computer
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Classification and specific primer design for accurate detection of SARS-CoV-2 using deep learning.

Scientific reports Jan 13, 2021
In this paper, deep learning is coupled with explainable artificial intelligence techniques for the discovery of representative genomic sequences in SARS-CoV-2. A convolutional neural network classifier is first trained on 553 sequences from the Nati...
Limit of Detection Polymerase Chain Reaction Deep Learning DNA Primers SARS-CoV-2
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Sensitive fluorescent detection of DNA methyltransferase using nicking endonuclease-mediated multiple primers-like rolling circle amplification.

Biosensors & bioelectronics Dec 30, 2016
Sensitive and reliable detection of DNA methyltransferase (MTase) is of great significance for both early tumor diagnosis and therapy. In this study, a simple, label-free and sensitive DNA MTase-sensing method was developed on the basis of a nicking ...
DNA (Cytosine-5-)-Methyltransferases Endonucleases G-Quadruplexes DNA-Cytosine Methylases Spectrometry, Fluorescence Humans HeLa Cells Nucleic Acid Amplification Techniques DNA (Cytosine-5-)-Methyltransferase 1 HEK293 Cells MCF-7 Cells DNA Primers Enzyme Assays DNA Biosensing Techniques
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Designing primers and evaluation of the efficiency of propidium monoazide - Quantitative polymerase chain reaction for counting the viable cells of Lactobacillus gasseri and Lactobacillus salivarius.

Journal of food and drug analysis Nov 29, 2016
The purpose of this study is to evaluate the efficiency of using propidium monoazide (PMA) real-time quantitative polymerase chain reaction (qPCR) to count the viable cells of Lactobacillus gasseri and Lactobacillus salivarius in probiotic products. ...
Polymerase Chain Reaction Azides Ligilactobacillus salivarius Real-Time Polymerase Chain Reaction Lactobacillus gasseri DNA Primers Bacterial Load Microbial Viability Lactobacillus Propidium DNA, Bacterial
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Estimation of teaching-learning-based optimization primer design using regression analysis for different melting temperature calculations.

IEEE transactions on nanobioscience Sep 12, 2014
Primers plays important role in polymerase chain reaction (PCR) experiments, thus it is necessary to select characteristic primers. Unfortunately, manual primer design manners are time-consuming and easy to get human negligence because many PCR const...
Polymerase Chain Reaction Analysis of Variance Transition Temperature DNA Base Sequence Humans Artificial Intelligence DNA Primers Regression Analysis Algorithms
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A Novel Teaching-Learning-Based Optimization for Improved Mutagenic Primer Design in Mismatch PCR-RFLP SNP Genotyping.

IEEE/ACM transactions on computational biology and bioinformatics Jan 1, 2016
Many single nucleotide polymorphisms (SNPs) for complex genetic diseases are genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in small-scale basic research studies. It is an essential work to design feasible ...
Computational Biology Supervised Machine Learning Polymerase Chain Reaction DNA Primers Polymorphism, Single Nucleotide Polymorphism, Restriction Fragment Length
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