AIMC Topic: Real-Time Polymerase Chain Reaction

Clear Filters Showing 1 to 10 of 56 articles

Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses.

Frontiers in cellular and infection microbiology Jan 28, 2025
INTRODUCTION: Viral calf diarrhea poses a significant challenge to the cattle industry worldwide due to its high morbidity and mortality rates, leading to substantial economic losses. The clinical symptoms associated with various diarrhea pathogens o...
Kobuvirus Multiplex Polymerase Chain Reaction Cattle Diseases Diarrhea DNA Primers Reproducibility of Results Feces Diarrhea Viruses, Bovine Viral Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Torovirus Real-Time Polymerase Chain Reaction Bovine Virus Diarrhea-Mucosal Disease Animals Cattle
View on PubMed DOI

Automated post-run analysis of arrayed quantitative PCR amplification curves using machine learning.

Gates open research Jan 20, 2025
BACKGROUND: The TaqMan Array Card (TAC) is an arrayed, high-throughput qPCR platform that can simultaneously detect multiple targets in a single reaction. However, the manual post-run analysis of TAC data is time consuming and subject to interpretati...
Humans Machine Learning Reproducibility of Results Sensitivity and Specificity Real-Time Polymerase Chain Reaction
View on PubMed DOI

A multi-class support vector machine classification model based on 14 microRNAs for forensic body fluid identification.

Forensic science international. Genetics Nov 21, 2024
MicroRNAs (miRNAs) are promising biomarkers for forensic body fluid identification owing to their small size, stability against degradation, and differential expression patterns. However, the expression of most body fluid-miRNAs is relative (differen...
Cervix Mucus Pericardial Fluid Saliva Body Fluids Forensic Genetics Menstruation Humans MicroRNAs Female Semen Real-Time Polymerase Chain Reaction Adult Genetic Markers Support Vector Machine Male Gene Expression Profiling
View on PubMed DOI

Integration of Machine Learning and Experimental Validation to Identify Anoikis-Related Prognostic Signature for Predicting the Breast Cancer Tumor Microenvironment and Treatment Response.

Genes Nov 12, 2024
Anoikis-related genes (ANRGs) are crucial in the invasion and metastasis of breast cancer (BC). The underlying role of ANRGs in the prognosis of breast cancer patients warrants further study. The anoikis-related prognostic signature (ANRS) was gene...
Breast Neoplasms Tumor Microenvironment Biomarkers, Tumor Middle Aged Prognosis Female Reproducibility of Results Aged Reverse Transcriptase Polymerase Chain Reaction Immunotherapy Enzyme-Linked Immunosorbent Assay Treatment Outcome Databases, Genetic Antineoplastic Agents Gene Expression Profiling Transcriptome Real-Time Polymerase Chain Reaction Machine Learning Imatinib Mesylate Predictive Value of Tests Proportional Hazards Models Drug Screening Assays, Antitumor Anoikis Gene Expression Blotting, Western
View on PubMed DOI

Enhancing SARS-CoV-2 Lineage Surveillance through the Integration of a Simple and Direct qPCR-Based Protocol Adaptation with Established Machine Learning Algorithms.

Analytical chemistry Nov 4, 2024
Emerging and evolving Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) lineages, adapted to changing epidemiological conditions, present unprecedented challenges to global public health systems. Here, we introduce an adapted analytical ap...
Humans Machine Learning COVID-19 SARS-CoV-2 Real-Time Polymerase Chain Reaction RNA, Viral
View on PubMed DOI

Harnessing machine learning technique to authenticate differentially expressed genes in oral squamous cell carcinoma.

Oral surgery, oral medicine, oral pathology and oral radiology Oct 17, 2024
OBJECTIVE: Advancements in early detection of the disease, prognosis and the development of therapeutic strategies necessitate tumor-specific biomarkers. Despite continuous efforts, no molecular marker has been proven to be an effective therapeutic t...
Male Middle Aged Gene Expression Profiling Real-Time Polymerase Chain Reaction Gene Expression Regulation, Neoplastic Predictive Value of Tests Mouth Neoplasms Female Carcinoma, Squamous Cell Aged Biomarkers, Tumor Humans Machine Learning Prognosis
View on PubMed DOI

Estimating epidemic trajectories of SARS-CoV-2 and influenza A virus based on wastewater monitoring and a novel machine learning algorithm.

The Science of the total environment Aug 27, 2024
The COVID-19 pandemic has altered the circulation of non-SARS-CoV-2 respiratory viruses. In this study, we carried out wastewater surveillance of SARS-CoV-2 and influenza A virus (IAV) in three key port cities in China through real-time quantitative ...
Machine Learning Algorithms SARS-CoV-2 Humans Influenza A virus Seasons China Wastewater-Based Epidemiological Monitoring Real-Time Polymerase Chain Reaction Influenza, Human
View on PubMed DOI

Identification and Validation of Aging Related Genes Signature in Chronic Obstructive Pulmonary Disease.

COPD Aug 13, 2024
PURPOSE: Chronic Obstructive Pulmonary Disease (COPD) is regarded as an accelerated aging disease. Aging-related genes in COPD are still poorly understood.
Class Ia Phosphatidylinositol 3-Kinase Biomarkers Pulmonary Disease, Chronic Obstructive Aging Gene Expression Profiling Gene Regulatory Networks Support Vector Machine Databases, Genetic Humans Real-Time Polymerase Chain Reaction Algorithms ROC Curve
View on PubMed DOI

Development, Validation, and Implementation of an Augmented Multiwell, Multitarget Quantitative PCR for the Analysis of Human Papillomavirus Genotyping through Software Automation, Data Science, and Artificial Intelligence.

The Journal of molecular diagnostics : JMD Jul 5, 2024
The value of human papillomavirus (HPV) testing for cervical cancer screening is well established; however, its use as a primary screening option or as a reflex test after atypical cytology results is now gaining wider acceptance. The importance of f...
Papillomavirus Infections DNA, Viral Genotyping Techniques Papillomaviridae Human Papillomavirus Viruses Uterine Cervical Neoplasms Viral Load Real-Time Polymerase Chain Reaction Genotype Artificial Intelligence Software Female Reproducibility of Results Humans
View on PubMed DOI

Developing an interpretation model for body fluid identification.

Forensic science international Apr 22, 2024
Criminal investigations, particularly sexual assaults, frequently require the identification of body fluid type in addition to body fluid donor to provide context. In most cases this can be achieved by conventional methods, however, in certain scenar...
Humans Bayes Theorem Menstruation Discriminant Analysis Body Fluids Cervix Mucus Semen Blood Chemical Analysis Saliva Machine Learning Female Male Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Logistic Models Models, Statistical
View on PubMed DOI
Popular Topics
Recent Journals