Molecular Visualization of Early-Stage Acute Kidney Injury with a DNA Framework Nanodevice.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Published Date: May 4, 2022

Abstract

DNA nanomachines with artificial intelligence have attracted great interest, which may open a new era of precision medicine. However, their in vivo behavior, including early diagnosis and therapeutic effect are limited by their targeting efficiency. Here, a tetrahedral DNA framework (TDF)-based nanodevice for in vivo near-infrared (NIR) diagnosis of early-stage AKI is developed. This nanodevice comprises three functional modules: a size-tunable TDF nanostructure as kidney-targeting vehicle, a binding module for the biomarker kidney injury molecule-1 (Kim-1), and a NIR signaling module. The cooperation of these modules allows the nanodevice to be selectively accumulated in injured kidney tissues with high Kim-1 level, generating strong NIR fluorescence; whereas the nanodevice with the proper size can be rapidly cleared in healthy kidneys to minimize the background. By using this nanodevice, the early diagnosis of AKI onset is demonstrated at least 6 h ahead of Kim-1 urinalysis, or 12 h ahead of blood detection. It is envisioned that this TDF-based nanodevice may have implications for the early diagnosis of AKI and other kidney diseases.

Authors

  • Fei Ding
    Information Processing and Communication Technology Lab, Shanghai Institute of Satellite Engineering, Shanghai, China.
  • Shuangye Zhang
    School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Suyu Liu
    Southern Medical University Affiliated Fengxian Hospital, The Second Affiliated Hospital of the Chinese University of Hong Kong (Shenzhen), Shenzhen, 518172, China.
  • Jing Feng
    Department of Urology, ZhongNan Hospital, Wuhan University, No. 169 Donghu Road, Wuhan, Hubei, 430071, China.
  • Jiang Li
  • Qian Li
    Emergency and Critical Care Center, Department of Emergency Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China.
  • Zhilei Ge
    School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China.
  • Xiaolei Zuo
    Institute of Molecular Medicine, Department of Liver Surgery, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
  • Chunhai Fan
    Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
  • Qiang Xia
    Department of Urology, Wuxi No. 9 People's Hospital Affiliated to Soochow University, Wuxi 214062, Jiangsu, China.

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