Optimization of Cultural Conditions for Cultured in Industrial Wastewater.
Journal:
BioMed research international
PMID:
30687758
Abstract
The aim of this study was to optimize the cultural conditions for using industrial wastewater as nature culture through response surface methodology. In our present study, we analyzed the total number of living in the fermentation broth using multispectral imaging flow cytometry. Plackett-Burman design was applied to evaluate the effects of six variables, namely, initial pH, industrial wastewater solubility, rotating speed, culture temperature, inoculum size, and loading volume. Loading volume, initial pH, and culture temperature were found to influence the biomass of significantly and were further optimized by Box-Behnken design. After verification test, the optimum fermentation conditions of using the processing wastewater as nature culture media were obtained as follows: initial pH of 7.4, culture temperature of 25°C, loading volume of 40 mL/250 mL, culture time of 24 h, industrial wastewater solubility of 1%, rotating speed of 200 rpm, and inoculum size of 8%. The predicted optimum model's value was 8.88 × 10 Obj/mL and the average experimental value was 9.03 ± 0.02 × 10 Obj/mL, which met the national microbial fertilizers' standard. Furthermore, the field experiment results showed that the fermentation broth of could significantly improve the yield of