Machine learning and biological evaluation-based identification of a potential MMP-9 inhibitor, effective against ovarian cancer cells SKOV3.

Journal: Journal of biomolecular structure & dynamics
Published Date:

Abstract

MMP-9, also known as gelatinase B, is a zinc-metalloproteinase family protein that plays a key role in the degradation of the extracellular matrix (ECM). The normal function of MMP-9 includes the breakdown of ECM, a process that aids in normal physiological processes such as embryonic development, angiogenesis, etc. Interruptions in these processes due to the over-expression or downregulation of MMP-9 are reported to cause some pathological conditions like neurodegenerative diseases and cancer. In the present study, an integrated approach for ML-based virtual screening of the Maybridge library was carried out and their biological activity was tested in an attempt to identify novel small molecule scaffolds that can inhibit the activity of MMP-9. The top hits were identified and selected for target-based activity against MMP-9 protein using the kit (Biovision K844). Further, MTT assay was performed in various cancer cell lines such as breast (MCF-7, MDA-MB-231), colorectal (HCT119, DL-D-1), cervical (HeLa), lung (A549) and ovarian cancer (SKOV3). Interestingly, one compound viz., RJF02215 exhibited anti-cancer activity selectively in SKOV3. Wound healing assay and colony formation assay performed on SKOV3 cell line in the presence of RJF02215 confirmed that the compound had a significant inhibitory effect on this cell line. Thus, we have identified a novel molecule that can inhibit MMP-9 activity and inhibits the proliferation of SKOV3 cells. Novel molecules based on the structure of RJF02215 may become a good value addition for the treatment of ovarian cancer by exhibiting selective MMP-9 activity.Communicated by Ramaswamy H. Sarma.

Authors

  • Khushboo Sinha
    Cancer Biology Division, CSIR-Central Drug Research Institute, Lucknow, India.
  • Shahid Parwez
    Biochemistry and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, India.
  • Shahana Mv
    Cancer Biology Division, CSIR-Central Drug Research Institute, Lucknow, India.
  • Ananya Yadav
    Cancer Biology Division, CSIR-Central Drug Research Institute, Lucknow, India.
  • Mohammad Imran Siddiqi
    Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow 226031, India. mi_siddiqi@cdri.res.in and Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Drug Research Institute, Campus, Lucknow 226031, India.
  • Dibyendu Banerjee
    Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, B.S. 10/1, Janakipuram Extension, Sitapur Road, Lucknow, 226031, India. d.banerjee@cdri.res.in and AcSIR (Academy of Scientific and Innovative Research), India.