Robotic cell processing facility for clinical research of retinal cell therapy.

Journal: SLAS technology
PMID: 39470449

Abstract

The consistent production of high-quality cells in cell therapy highlights the potential of automated manufacturing. Humanoid robots are a useful option for transferring technology to automate human cell cultures. This study evaluated a robotic cell-processing facility (R-CPF) for clinical research on retinal cell therapy, incorporating the versatile humanoid robot Maholo LabDroid and an All-in-One CP unit. The R-CPF platform consists of a robot area for handling cells and an operator area for the maintenance of the robot, designed with a clean airflow to ensure sterility. Monitoring the falling, floating, and adhering bacteria demonstrated that the required cleanliness and aseptic environment for cell manufacturing were satisfied. We then conducted cell manufacturing equivalent to the transplantation therapy of induced pluripotent stem cell (iPSC)-derived retinal pigment epithelial cells that met the clinical quality standards for transplantation. These results indicate that R-CPF is suitable for cell manufacturing purposes and suggest that utilizing the same robotic system in basic and clinical research can accelerate the translation of basic research findings into clinical applications.

Authors

  • Motoki Terada
    VCCT Inc. Kobe Eye Center Building 5F, 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan; Kobe City Eye Hospital. 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047 Japan.
  • Yu Kogawa
    Technical Research Laboratory Innovation Division, DAI-DAN Co., Ltd. 390 Kitanagai, Miyoshi-cho, Iruma-gun, Saitama 354-0044 Japan.
  • Yumiko Shibata
    VCCT Inc. Kobe Eye Center Building 5F, 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan; Kobe City Eye Hospital. 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047 Japan.
  • Michinori Kitagawa
    VCCT Inc. Kobe Eye Center Building 5F, 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan.
  • Shinya Kato
    Dept. of Surgery, National Hospital Organization, Osaka National Hospital.
  • Tomomitsu Iida
    VCCT Inc. Kobe Eye Center Building 5F, 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan; Kobe City Eye Hospital. 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047 Japan.
  • Tsuyoshi Yorimitsu
    Technical Research Laboratory Innovation Division, DAI-DAN Co., Ltd. 390 Kitanagai, Miyoshi-cho, Iruma-gun, Saitama 354-0044 Japan.
  • Akari Kato
    Laboratory for Biologically Inspired Computing, RIKEN Center for Biosystems Dynamics Research. 6-2-3 Furuedai, Suita, Osaka 565-0874 Japan.
  • Kenji Matsukuma
    Robotic Biology Institute Inc. Telecom Center Building East Wing 1F, 2-5-10 Aomi, Koto-ku, Tokyo 135-0064 Japan.
  • Tadao Maeda
    Research Center, Kobe City Eye Hospital, Kobe, Japan.
  • Masayo Takahashi
  • Genki N Kanda
    Kobe City Eye Hospital. 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047 Japan; Robotic Biology Institute Inc. Telecom Center Building East Wing 1F, 2-5-10 Aomi, Koto-ku, Tokyo 135-0064 Japan; Laboratory for Biologically Inspired Computing, RIKEN Center for Biosystems Dynamics Research. 6-2-3 Furuedai, Suita, Osaka 565-0874 Japan; Vision Care Inc. Kobe Eye Center Building 5F, 2-1-8 Minatojima Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047 Japan. Electronic address: genki.kanda@riken.jp.

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