Effect of laser photobiomodulation combined with hydroxyapatite nanoparticles on the osteogenic differentiation of mesenchymal stem cells using artificial intelligence: An in vitro study.

Journal: PloS one
PMID:

Abstract

AIM: To evaluate in vitro the effect of laser photobiomodulation (PBM) combined or not with 30-nm hydroxyapatite nanoparticles (HANp), on the osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) by morphometric analysis using artificial intelligence programs (TensorFlow and ArcGIS).

Authors

  • Eloiza Leonardo de Melo
    Department of Biophotonics in Health Sciences, University of Pernambuco, Recife, Pernambuco, Brazil.
  • Jéssica Meirinhos Miranda
    Department of Biophotonics in Health Sciences, University of Pernambuco, Recife, Pernambuco, Brazil.
  • Vanessa Bastos de Souza Rolim Lima
    Department of Biophotonics in Health Sciences, University of Pernambuco, Recife, Pernambuco, Brazil.
  • Wyndly Daniel Cardoso Gaião
    Department of Biophysics and Radiobiology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
  • Braulio de Vilhena Amorim Tostes
    Department of Basic Chemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
  • Claudio Gabriel Rodrigues
    Department of Biophysics and Radiobiology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
  • Márcia Bezerra da Silva
    Department of Biophysics and Radiobiology, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
  • Severino Alves Júnior
    Department of Basic Chemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
  • Edson Luiz Pontes Perger
    Department of Bioprocess and Biotechnology, Paulista State University, Botucatu, São Paulo, Brazil.
  • Mávio Eduardo Azevedo Bispo
    Department of Computer Science, University of Pernambuco, Recife, Pernambuco, Brazil.
  • Marleny Elizabeth Márquez de Martínez Gerbi
    Department of Biophotonics in Health Sciences, University of Pernambuco, Recife, Pernambuco, Brazil.