Procyanidin A2 in the Australian plant Alectryon oleifolius has anthelmintic activity against equine cyathostomins in vitro.

Journal: Veterinary parasitology
PMID: 29279088

Abstract

There is a need to investigate new methods of controlling cyathostomins in horses due to increasing anthelmintic resistance amongst these parasites. In a previous study we identified the Australian plant Alectryon oleifolius as having anthelmintic activity towards cyathostomins. This study aimed to isolate and identify the bioactive compound(s) responsible for all or part of this anthelmintic activity and quantify its activity in vitro. The condensed tannin procyanidin A2 was isolated from the plant through a process of bioassay guided fractionation and identified using 1D and 2D nuclear magnetic resonance spectroscopy and high performance liquid chromatography with mass spectrometry. Procyanidin A2 demonstrated significant anthelmintic activity in larval development assays, completely inhibiting development from egg to third larval stage at concentrations as low as 50μg/mL and having an IC value of 12.6μg/mL. Procyanidin A2 also significantly inhibited larval migration at concentrations of 25μg/mL. This study indicates that procyanidin A2 is the principal anthelmintic compound in extracts from A. oleifolius, and further highlights the potential for the use of this plant as a component of cyathostomin control programs in the future.

Authors

  • S E Payne
    School of Agriculture and Environment, The University of Western Australia, Crawley, Australia; Institute of Agriculture, The University of Western Australia, Crawley, Australia. Electronic address: stephanie.payne@uwa.edu.au.
  • G R Flematti
    School of Molecular Sciences, The University of Western Australia, Crawley, Australia.
  • A Reeder
    Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Crawley, Australia.
  • A C Kotze
    CSIRO Agriculture and Food, Queensland Bioscience Precinct, University of Queensland, Brisbane, Australia.
  • Z Durmic
    School of Agriculture and Environment, The University of Western Australia, Crawley, Australia; Institute of Agriculture, The University of Western Australia, Crawley, Australia.
  • P E Vercoe
    School of Agriculture and Environment, The University of Western Australia, Crawley, Australia; Institute of Agriculture, The University of Western Australia, Crawley, Australia.

Keywords

External Resources

Popular Topics
Recent Journals