AIEgen-deep: Deep learning of single AIEgen-imaging pattern for cancer cell discrimination and preclinical diagnosis.

Journal: Biosensors & bioelectronics
Published Date:

Abstract

This study introduces AIEgen-Deep, an innovative classification program combining AIEgen fluorescent dyes, deep learning algorithms, and the Segment Anything Model (SAM) for accurate cancer cell identification. Our approach significantly reduces manual annotation efforts by 80%-90%. AIEgen-Deep demonstrates remarkable accuracy in recognizing cancer cell morphology, achieving a 75.9% accuracy rate across 26,693 images of eight different cell types. In binary classifications of healthy versus cancerous cells, it shows enhanced performance with an accuracy of 88.3% and a recall rate of 79.9%. The model effectively distinguishes between healthy cells (fibroblast and WBC) and various cancer cells (breast, bladder, and mesothelial), with accuracies of 89.0%, 88.6%, and 83.1%, respectively. Our method's broad applicability across different cancer types is anticipated to significantly contribute to early cancer detection and improve patient survival rates.

Authors

  • Haojun Hua
    City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, 999077, China.
  • Yanlin Deng
    City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, 999077, China.
  • Jing Zhang
    MOEMIL Laboratory, School of Optoelectronic Information, University of Electronic Science and Technology of China, Chengdu, China.
  • Xiang Zhou
    Department of Sociology, Harvard University, Cambridge, Massachusetts, USA.
  • Tianfu Zhang
    School of Biomedical Engineering, Guangzhou Medical University, Guangzhou, China. Electronic address: zhangtf@gzhmu.edu.cn.
  • Bee Luan Khoo
    City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, 999077, China; Hong Kong Center for Cerebro-Cardiovascular Health Engineering (COCHE), Hong Kong SAR, China; Department of Precision Diagnostic and Therapeutic Technology, City University of Hong Kong, Futian-Shenzhen Research Institute, Shenzhen 518057, China. Electronic address: blkhoo@cityu.edu.hk.