The Essential Oils of Hairy Roots and Roots of Soil-Grown Plants: Chemical Composition and Antimicrobial, Anti-Inflammatory, and Antioxidant Activities.

Journal: Oxidative medicine and cellular longevity
PMID: 28074117

Abstract

The essential oils were isolated by hydrodistillation from the hairy roots (HR) and roots of soil-grown plants (SGR) of and were analyzed by GC-MS method. In the both essential oils 62 compounds were identified. The root essential oils showed the differences in the qualitative and quantitative composition. The sesquiterpene hydrocarbons (55-62%) dominated in both essential oils. The major compounds of HR essential oil were cyperene, 13-norcypera-1(5),11(12)-diene, and cadalene while aplotaxene, nardosina-1(10),11-diene, and dauca-4(11),8-diene dominated in SGR essential oil. Both essential oils showed antibacterial activity especially against (ATCC 29212) and (ATCC 27853) (MIC value = 125 g/mL). HR and SGR essential oils also decreased the expression of IL-1, IL-6, and TNF- and the ROS level in LPS-treatment astrocytes. This is the first report to describe the chemical composition of essential oil from hairy roots, its protective effect against LPS-induced inflammation and ROS production in astrocytes, and its antimicrobial potential. The results show that hairy roots may be a valuable source of the essential oil and may be an alternative to the roots of soil-grown plants.

Authors

  • Ewa Skała
    Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland.
  • Patrícia Rijo
    Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, 1649-003 Lisboa, Portugal; Center for Research in Biosciences & Health Technologies (CBIOS), Universidade Lusófona de Humanidades e Tecnologias, 1749-024 Lisboa, Portugal.
  • Catarina Garcia
    Center for Research in Biosciences & Health Technologies (CBIOS), Universidade Lusófona de Humanidades e Tecnologias, 1749-024 Lisboa, Portugal.
  • Przemysław Sitarek
    Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland.
  • Danuta Kalemba
    Institute of General Food Chemistry, Lodz University of Technology, Stefanowskiego 4/10, 90-924 Lodz, Poland.
  • Monika Toma
    Department of Molecular Genetics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
  • Janusz Szemraj
    Department of Medical Biochemistry, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland.
  • Dariusz Pytel
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Hollings Cancer Center, HCC-709, 86 Jonathan Lucas Street, Charleston, SC 29425, USA.
  • Halina Wysokińska
    Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland.
  • Tomasz Śliwiński
    Department of Molecular Genetics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.

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