Alkaline phosphatase determines polyphosphate-induced mineralization in a cell-type independent manner.

Journal: Journal of bone and mineral metabolism
PMID: 26475372

Abstract

Polyphosphate [Poly(P)] has positive effects on osteoblast mineralization; however, the underlying mechanism remains unclear. In addition, it is unknown whether Poly(P) promotes mineralization in soft tissues. We investigated this by using various cells. Poly(P) concentrations of 1 and 0.5 mg/mL yielded high levels of mineralization in ROS17/2.8 osteoblast cells. Similarly, Poly(P) induced mineralization in cell types expressing alkaline phosphatase (ALP), namely, ATDC5 and MC3T3-E1, but not in CHO, C3H10T1/2, C2C12, and 3T3-L1 cells. Furthermore, forced expression of ALP caused Poly(P)-induced mineralization in CHO cells. These results suggest that ALP determines Poly(P)-induced mineralization in a cell-type independent manner.

Authors

  • Yoshikazu Mikami
    Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
  • Hiromasa Tsuda
    Department of Biochemistry, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan. tsuda.hiromasa@nihon-u.ac.jp.
  • Yuko Akiyama
    Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
  • Masaki Honda
    Department of Oral Anatomy, Aichi-Gakuin University School of Dentistry, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 464-8650, Japan.
  • Noriyoshi Shimizu
    Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
  • Naoto Suzuki
    Department of Biochemistry, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
  • Kazuo Komiyama
    Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.

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