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Deep Eutectic Solvent Extraction Assisted Ligand Affinity Assay for α-Glucosidase Inhibitors Screening From the Plasma of Rats Administrated Pueraria lobata Extracts.

Journal of separation science
In this work, for the first time, a deep eutectic solvent assisted ligand affinity assay was proposed. Several critical parameters affecting the analysis performance were investigated and the optimized DES extract conditions were as follows: the solu...

Impact of dental pulp cells-derived small extracellular vesicles on the properties and behavior of dental pulp cells: an in-vitro study.

BMC oral health
BACKGROUND: Dental pulp cells-derived small extracellular vesicles (DPCs-sEVs) had shown immunomodulatory, anti-inflammatory, and tissue function restorative abilities. Therefore, DPCs-sEVs should be considered as a promising regenerative tool for de...

Amelioration of Chronic Ethanol Administration-Induced Learning and Memory Impairments by High-Intensity Interval Training (HIIT) and Ritalin.

Brain and behavior
OBJECTIVES: The current study aimed to investigate the impacts of 8-week high-intensity interval training (HIIT) and Ritalin (RIT), alone and in combination, on cognitive functions and hippocampal oxidative parameters following chronic ethanol consum...

Altered aminoacid and lipid metabolism in a rat orofacial inflammation model determined by omics approach: potential role in trigeminal sensitisation.

The journal of headache and pain
BACKGROUND: Trigeminal activation and sensitisation involved in chronic inflammatory orofacial pain share several similarities with headaches, including migraine. Therefore, understanding the pathophysiological mechanisms is important to determine no...

Gui-Pi-Tang Defers Skeletal Muscle and Cardiac Muscle Aging by Promoting Mitochondrial Remodeling.

Drug design, development and therapy
PURPOSE: To determine whether Gui-Pi-Tang (GPT) has protective effects on skeletal muscle and cardiac muscle in aged mice.

Reported liver toxicity of food chemicals in rats extrapolated to humans using virtual human-to-rat hepatic concentration ratios generated by pharmacokinetic modeling with machine learning-derived parameters.

The Journal of toxicological sciences
Pharmacokinetic data are not generally available for evaluating the toxicological potential of food chemicals. A simplified physiologically based pharmacokinetic (PBPK) model has been established to evaluate internal exposures to chemicals in rats or...

Machine learning and complex network analysis of drug effects on neuronal microelectrode biosensor data.

Scientific reports
Biosensors, such as microelectrode arrays that record in vitro neuronal activity, provide powerful platforms for studying neuroactive substances. This study presents a machine learning workflow to analyze drug-induced changes in neuronal biosensor da...

Identification of pivotal genes and regulatory networks associated with SAH based on multi-omics analysis and machine learning.

Scientific reports
Subarachnoid hemorrhage (SAH) is a disease with high mortality and morbidity, and its pathophysiology is complex but poorly understood. To investigate the potential therapeutic targets post-SAH, the SAH-related feature genes were screened by the comb...

Repetitive training enhances the pattern recognition capability of cultured neural networks.

PLoS computational biology
Cultured neural networks in vitro have demonstrated the biocomputing capability to recognize patterns. However, the underlying mechanisms behind information processing and pattern recognition remain less understood. Here, we developed an in vitro neu...

Virtual white matter: a novel system for cross-dish neural interaction and modulation.

Journal of neural engineering
. Biological neural networks (BNNs) are characterized by complex interregional connectivity, allowing for seamless communication between different brain regions.models traditionally consist of single-dish neural cultures that cannot recapitulate the ...