Modulatory role of garlicin in migration and invasion of intrahepatic cholangiocarcinoma via PI3K/AKT pathway.
Journal:
International journal of clinical and experimental pathology
PMID:
26823715
Abstract
Increasing evidences have indicated the role of garlicin in inhibiting the progression of various tumors including glioma, pulmonary carcinoma and pancreatic carcinoma, via mediating cell apoptosis or cell cycle. The regulatory effect and related molecular mechanism of garlicin in intrahepatic cholangiocarcinoma, however, remained unknown. This study thus aimed to investigate this scientific issue. HCCC-9810 cell line was treated with serially diluted garlicin, followed by cell proliferation assay using MTT approach. Transwell migration and invasion assays were further employed the regulatory effect of garlicin. The expression level of p-AKT and AKT proteins in tumor cells was quantified by Western blot. The growth of tumor cells was significantly inhibited by high concentration of garlicin (> 1.5 μM). Lower concentration of garlicin showed dose-dependent inhibition of tumor cell invasion and migration. After using specific agonist IGF-1 (50 ng/mL) of PI3K/AKT signaling pathway, such facilitating effects of garlicin were depressed (P < 0.05). Western blotting showed significantly decreased phosphorylation level of AKT after treated with gradient concentrations of garlicin, while leaving the total AKT protein level unchanged. Garlicin may inhibit the invasion and migration of intrahepatic cholangiocarcinoma cells via inhibiting PI3K/AKT signaling pathway.
Authors
Keywords
Allyl Compounds
Antineoplastic Agents, Phytogenic
Bile Duct Neoplasms
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cholangiocarcinoma
Disulfides
Dose-Response Relationship, Drug
Humans
Neoplasm Invasiveness
Phosphatidylinositol 3-Kinase
Phosphoinositide-3 Kinase Inhibitors
Phosphorylation
Protein Kinase Inhibitors
Proto-Oncogene Proteins c-akt
Signal Transduction