Differentiation of glioblastoma G4 and two types of meningiomas using FTIR spectra and machine learning.

Journal: Analytical biochemistry
PMID:

Abstract

Brain tumors are among the most dangerous, due to their location in the organ that governs all life processes. Moreover, the high differentiation of these poses a challenge in diagnostics. Therefore, this study focused on the chemical differentiation of glioblastoma G4 (GBM) and two types of meningiomas (atypical - MAtyp and angiomatous - MAng) were done using Fourier Transform InfraRed (FTIR) spectroscopy, combined with statistical, multivariate, machine learning and rate of spectrum changes methods. The positions of all analyzed peaks differed between GBM and meningiomas. However, for two types of meningiomas, only shift of peaks corresponding to CH bending vibrations, symmetric stretching vibrations of CH amide A, amide I, CO lipids vibrations, asymmetric stretching vibrations of CH were observed. Principal Component Analysis showed clear differentiation between GBM and the meningiomas. Decision tree clearly showed that wavenumbers corresponding to CO lipids vibrations provided the highest differentiation between GBM and meningiomas tissues, while amide I for two types of meningiomas. The accuracy and specificity of the results for GBM and meningiomas were more than 90 %, while for MAtyp and MAng, these parameters were around 80 %.

Authors

  • Bartłomiej Tołpa
    Department of Neurosurgery, Clinical Hospital No 2 in Rzeszow, Poland. Electronic address: bart.tolpa@gmail.com.
  • Wiesław Paja
    Faculty of Mathematics and Natural Sciences, University of Rzeszów, Rzeszów, Poland.
  • Pawel Jakubczyk
    Institute of Physics, College of Natural Sciences, University of Rzeszow, PL-35959 Rzeszow, Poland.
  • Kornelia Łach
    Clinic of Paediatric Oncology and Haematology, Faculty of Medicine, University of Rzeszow, ul. Kopisto 2a, 35-310 Rzeszow, Poland. kornelia_lach@wp.pl.
  • Elżbieta Trojnar
    Clinical Department of Pathomorphology, Clinical Hospital No 2 Rzeszow, Poland.
  • Agnieszka Gala-Błądzińska
    Institute of Medical Sciences, Medical College of Rzeszow University, Rzeszow, Poland.
  • Aneta Kowal
    Doctoral School, Institute of Medical Sciences, University of Rzeszow, Poland.
  • Bartosz Klebowski
    Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland.
  • Józef Cebulski
    Center for Innovation and Transfer of Natural Sciences and Engineering Knowledge, University of Rzeszow, 35-959 Rzeszow, Poland. cebulski@ur.edu.pl.
  • Joanna Depciuch
    Institute of Nuclear Physics, Polish Academy of Sciences, 31-342 Krakow, Poland. joannadepciuch@gmail.com.