Robot-Assisted Stereotactic Microinjection Method for Precision Cell Transplantation in Rat and Canine Models.

Journal: Cell transplantation
PMID: 40103182

Abstract

Cell transplantation is a promising approach for addressing neurodegenerative conditions. In this study, we developed a robot-assisted stereotactic microinjection system for transplanting cells. We evaluated the factors that affect cellular graft viability and other properties, including the gauge of the syringe needle and the injection rate. We systematically compared the synchronous withdrawal injection (SWI) and fixed-point injection (FPI) procedures in agarose and rat brain models. assessments revealed superior dye dispersion with SWI compared to FPI, and analyses confirmed that SWI reduced the tissue injury and improved cell distribution in the striatum. We applied this robot-assisted technique to evaluate the accuracy and safety of cell transplantation in canine models. Overall, this strategy enhances the accuracy and safety of graft delivery, potentially improving outcomes and advancing therapeutic strategies for the clinical treatment of neurodegenerative disorders.

Authors

  • Deqiang Han
    Cell Therapy Center, Beijing Municipal Geriatric Medical Research Center, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Xuanwu Hospital Capital Medical University, Beijing, China.
  • Sichang Chen
    Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China; China International Neuroscience Institute (CHINA-INI), Beijing, China.
  • Yuan Wang
    State Key Laboratory of Soil and Sustainable Agriculture, Changshu National Agro-Ecosystem Observation and Research Station, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.
  • Xueyao Wang
    Cell Therapy Center, Beijing Municipal Geriatric Medical Research Center, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Xuanwu Hospital Capital Medical University, Beijing, China.
  • Xingzhe Wang
    Cell Therapy Center, Beijing Municipal Geriatric Medical Research Center, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Xuanwu Hospital Capital Medical University, Beijing, China.
  • Tianqi Zheng
    Cell Therapy Center, Beijing Municipal Geriatric Medical Research Center, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Xuanwu Hospital Capital Medical University, Beijing, China.
  • Zhiguo Chen
    Cell Therapy Center, Beijing Municipal Geriatric Medical Research Center, National Clinical Research Center for Geriatric Diseases, and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Xuanwu Hospital Capital Medical University, Beijing, China.

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