Wide-field spontaneous Raman spectroscopy imaging system for biological tissue interrogation.
Journal:
Optics letters
Published Date:
Oct 15, 2016
Abstract
Raman spectroscopy has shown great promise as a method to discriminate between cancerous and normal tissue/cells for a range of oncology applications using microscopy and tissue interrogation instruments such as handheld probes and needles. Here we are presenting preliminary steps toward the development of a practical handheld macroscopic Raman spectroscopy instrument, demonstrating its capabilities to discriminate between different biological tissue types during ex vivo porcine experiments. The novel probe design can image a field of view of 25 mm with a spatial resolution <100 μm and an average spectral resolution of 95 cm, covering the fingerprint region between 450 to 1750 cm. The ability of the system to produce tissue maps based on molecular characteristics is demonstrated using a neural network machine learning technique.