CellSort: a support vector machine tool for optimizing fluorescence-activated cell sorting and reducing experimental effort.

Journal: Bioinformatics (Oxford, England)
Published Date: Mar 15, 2017

Abstract

MOTIVATION: High throughput screening by fluorescence activated cell sorting (FACS) is a common task in protein engineering and directed evolution. It can also be a rate-limiting step if high false positive or negative rates necessitate multiple rounds of enrichment. Current FACS software requires the user to define sorting gates by intuition and is practically limited to two dimensions. In cases when multiple rounds of enrichment are required, the software cannot forecast the enrichment effort required.

Authors

  • Jessica S Yu
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
  • Dante A Pertusi
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
  • Adebola V Adeniran
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
  • Keith E J Tyo
    Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.

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