An integrated molecular diagnostic report for heart transplant biopsies using an ensemble of diagnostic algorithms.

Journal: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation
Published Date: Jun 1, 2019

Abstract

BACKGROUND: We previously reported a microarray-based diagnostic system for heart transplant endomyocardial biopsies (EMBs), using either 3-archetype (3AA) or 4-archetype (4AA) unsupervised algorithms to estimate rejection. In the present study we examined the stability of machine-learning algorithms in new biopsies, compared 3AA vs 4AA algorithms, assessed supervised binary classifiers trained on histologic or molecular diagnoses, created a report combining many scores into an ensemble of estimates, and examined possible automated sign-outs.

Authors

  • Michael D Parkes
    Alberta Transplant Applied Genomics Centre, University of Alberta, Edmonton, Alberta, Canada.
  • Arezu Z Aliabadi
    Department of Cardiac Surgery, Medical University of Vienna, Vienna, Austria.
  • Martin Cadeiras
    Ronald Reagan UCLA Medical Center, Los Angeles, California, USA.
  • Maria G Crespo-Leiro
    Complexo Hospitalario Universitario A Coruña (CHUAC)-CIBERCV, A Coruña, Spain.
  • Mario Deng
    Ronald Reagan UCLA Medical Center, Los Angeles, California, USA.
  • Eugene C Depasquale
    Ronald Reagan UCLA Medical Center, Los Angeles, California, USA.
  • Johannes Goekler
    Department of Cardiac Surgery, Medical University of Vienna, Vienna, Austria.
  • Daniel H Kim
    Department of Neurosurgery, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX, 77030, USA.
  • Jon Kobashigawa
    Cedars-Sinai Medical Center, Beverly Hills, California, USA.
  • Alexandre Loupy
    Hôpital Necker, Paris, France.
  • Peter Macdonald
    The Victor Chang Cardiac Research Institute, Sydney, New South Wales, Australia.
  • Luciano Potena
    Cardiovascular Department, University of Bologna, Bologna, Italy.
  • Andreas Zuckermann
    Department of Cardiac Surgery, Medical University of Vienna, Vienna, Austria.
  • Philip F Halloran
    Alberta Transplant Applied Genomics Centre, University of Alberta, Edmonton, Alberta, Canada; Department of Medicine, University of Alberta, Edmonton, Alberta, Canada. Electronic address: phallora@ualberta.ca.

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