A simple Ca-imaging approach to neural network analyses in cultured neurons.
Journal:
Journal of neuroscience methods
Published Date:
Dec 17, 2020
Abstract
BACKGROUND: Ca-imaging is a powerful tool to measure neuronal dynamics and network activity. To monitor network-level changes in cultured neurons, neuronal activity is often evoked by electrical or optogenetic stimulation and assessed using multi-electrode arrays or sophisticated imaging. Although such approaches allow detailed network analyses, multi-electrode arrays lack single-cell precision, whereas optical physiology generally requires advanced instrumentation that may not be universally available.