Three-Dimensional Visualization of the Podocyte Actin Network Using Integrated Membrane Extraction, Electron Microscopy, and Machine Learning.

Journal: Journal of the American Society of Nephrology : JASN
PMID: 34758982

Abstract

BACKGROUND: Actin stress fibers are abundant in cultured cells, but little is known about them . In podocytes, much evidence suggests that mechanobiologic mechanisms underlie podocyte shape and adhesion in health and in injury, with structural changes to actin stress fibers potentially responsible for pathologic changes to cell morphology. However, this hypothesis is difficult to rigorously test due to challenges with visualization. A technology to image the actin cytoskeleton at high resolution is needed to better understand the role of structures such as actin stress fibers in podocytes.

Authors

  • Chengqing Qu
    Department of Mechanical Engineering, National Science Foundation Science and Technology Center for Engineering Mechanobiology, Washington University in St. Louis, St. Louis, Missouri.
  • Robyn Roth
    Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri.
  • Pongpratch Puapatanakul
    Division of Nephrology, Washington University School of Medicine, St. Louis, Missouri.
  • Charles Loitman
    Division of Nephrology, Washington University School of Medicine, St. Louis, Missouri.
  • Dina Hammad
    Division of Nephrology, Washington University School of Medicine, St. Louis, Missouri.
  • Guy M Genin
    Department of Mechanical Engineering, National Science Foundation Science and Technology Center for Engineering Mechanobiology, Washington University in St. Louis, St. Louis, Missouri.
  • Jeffrey H Miner
    Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri.
  • Hani Y Suleiman
    Division of Nephrology, Washington University School of Medicine, St. Louis, Missouri.

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