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Actin Cytoskeleton

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Machine-Learning-Based Approach to Decode the Influence of Nanomaterial Properties on Their Interaction with Cells.

ACS applied materials & interfaces
In an nanotoxicity system, cell-nanoparticle (NP) interaction leads to the surface adsorption, uptake, and changes into nuclei/cell phenotype and chemistry, as an indicator of oxidative stress, genotoxicity, and carcinogenicity. Different types of n...

Network Analysis of Integrin Adhesion Complexes.

Methods in molecular biology (Clifton, N.J.)
Cell-surface adhesion receptors mediate interactions with the extracellular matrix (ECM) to control many fundamental aspects of cell behavior, including cell migration, survival, and proliferation. Integrin adhesion receptors recruit structural and s...

Three-Dimensional Visualization of the Podocyte Actin Network Using Integrated Membrane Extraction, Electron Microscopy, and Machine Learning.

Journal of the American Society of Nephrology : JASN
BACKGROUND: Actin stress fibers are abundant in cultured cells, but little is known about them . In podocytes, much evidence suggests that mechanobiologic mechanisms underlie podocyte shape and adhesion in health and in injury, with structural change...

Live-Cell Labeling and Artificial Intelligence Approaches for High-Resolution XYZT Imaging of Cytoskeletal Dynamics During Collective Cell Migration.

Methods in molecular biology (Clifton, N.J.)
Collective cell migration is crucial for a variety of pathophysiological processes including embryonic development, wound healing, carcinoma invasion, and sprouting angiogenesis. The behavior of leading and following cells during migration is highly ...

A deep learning framework for quantitative analysis of actin microridges.

NPJ systems biology and applications
Microridges are evolutionarily conserved actin-rich protrusions present on the apical surface of squamous epithelial cells. In zebrafish epidermal cells, microridges form self-evolving patterns due to the underlying actomyosin network dynamics. Howev...

Spatiotemporal feature learning for actin dynamics.

PloS one
The social amoeba Dictyostelium discoideum is a standard model system for studying cell motility and formation of biological patterns. D. discoideum cells form protrusions and migrate via cytoskeletal reorganization driven by coordinated waves of act...

Distinct actin microfilament localization during early cell plate formation through deep learning-based image restoration.

Plant cell reports
Using deep learning-based image restoration, we achieved high-resolution 4D imaging with minimal photodamage, revealing distinct localization and suggesting Lifeact-RFP-labeled actin microfilaments play a role in initiating cell plate formation. Phra...