Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report.

Journal: Acta medica (Hradec Kralove)
PMID: 32036851

Abstract

A significant population of ultrashort (50-150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL-1), but not in plasma of healthy donors. An original high resolution HPLC technique has been proposed to reveal and characterize this peculiarity. To solve this task, a novel molecular size exclusion - anion exchange analytical technique was developed. Its applicability to diagnostics and oncogenesis research is quizzed here.

Authors

  • Kirill V Ermakov
    N.I. Pirogov Russian National Research Medical University, Russian Federal Ministry of Health, Ostrovityanov St., 1, Moscow 117997, Russia. ermakovkv07@gmail.com.
  • Alexander A Bukhvostov
    N.I. Pirogov Russian National Research Medical University, Russian Federal Ministry of Health, Ostrovityanov St., 1, Moscow 117997, Russia.
  • Alexander S Vedenkin
    N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Kosygin St., 4, Moscow 119991, Russia.
  • Sergey V Stovbun
    N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Kosygin St., 4, Moscow 119991, Russia.
  • Anton S Dvornikov
    N.I. Pirogov Russian National Research Medical University, Russian Federal Ministry of Health, Ostrovityanov St., 1, Moscow 117997, Russia.
  • Dmitry A Kuznetsov
    N.I. Pirogov Russian National Research Medical University, Russian Federal Ministry of Health, Ostrovityanov St., 1, Moscow 117997, Russia.

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