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DNA, Single-Stranded

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A tri-functional probe mediated exponential amplification strategy for highly sensitive detection of Dnmt1 and UDG activities at single-cell level.

Analytica chimica acta
Multiplex DNA methylation and glycosylation are ubiquitous in the human body to ensure the normal function and stability of the genome. The methyltransferases and glycosylases rely on varied enzymes with different action mechanism, which still remain...

Ultrashort ssDNA in Retinoblastoma Patients Blood Plasma Detected by a Novel High Resolution HPLC Technique: a Preliminary Report.

Acta medica (Hradec Kralove)
A significant population of ultrashort (50-150n) single-stranded DNA fragments were found in exosome-free blood plasma of retinoblastoma patients (6.84 ng mL-1), but not in plasma of healthy donors. An original high resolution HPLC technique has been...

Automated Stoichiometry Analysis of Single-Molecule Fluorescence Imaging Traces via Deep Learning.

Journal of the American Chemical Society
The stoichiometry of protein complexes is precisely regulated in cells and is fundamental to protein function. Singe-molecule fluorescence imaging based photobleaching event counting is a new approach for protein stoichiometry determination under phy...

In silico screening of ssDNA aptamer against Escherichia coli O157:H7: A machine learning and the Pseudo K-tuple nucleotide composition based approach.

Computational biology and chemistry
This study was planned to in silico screening of ssDNA aptamer against Escherichia coli O157:H7 by combination of machine learning and the PseKNC approach. For this, firstly a total numbers of 47 validated ssDNA aptamers as well as 498 random DNA seq...

Discrimination of Genetic Biomarkers of Disease through Machine-Learning-Based Hypothesis Testing of Direct SERS Spectra of DNA and RNA.

ACS sensors
Cancer is globally a leading cause of death that would benefit from diagnostic approaches detecting it in its early stages. However, despite much research and investment, cancer early diagnosis is still underdeveloped. Owing to its high sensitivity, ...

Deep learning-based spectroscopic single-molecule localization microscopy.

Journal of biomedical optics
SIGNIFICANCE: Spectroscopic single-molecule localization microscopy (sSMLM) takes advantage of nanoscopy and spectroscopy, enabling sub-10 nm resolution as well as simultaneous multicolor imaging of multi-labeled samples. Reconstruction of raw sSMLM ...

Machine learning supported single-stranded DNA sensor array for multiple foodborne pathogenic and spoilage bacteria identification in milk.

Food chemistry
Ensuring food safety through rapid and accurate detection of pathogenic bacteria in food products is a critical challenge in the food supply chain. In this study, a non-specific optical sensor array was proposed for the identification of multiple pat...

Rewireable Building Blocks for Enzyme-Powered DNA Computing Networks.

Journal of the American Chemical Society
Neural networks enable the processing of large, complex data sets with applications in disease diagnosis, cell profiling, and drug discovery. Beyond electronic computers, neural networks have been implemented using programmable biomolecules such as D...

A novel four-modal nano-sensor based on two-dimensional Mxenes and fully connected artificial neural networks for the highly sensitive and rapid detection of ochratoxin A.

Talanta
Timely and accurate on-site detection of ochratoxin A (OTA) is extremely important for global public health. In this study, a fluorescence/colorimetric biosensor based on TiC nano-materials (TiC-NMS) and a machine-learning (ML) based fluorescence/col...

Deep Learning-Assisted Fluorescence Single-Particle Detection of Fumonisin B Powered by Entropy-Driven Catalysis and Argonaute.

Analytical chemistry
Timely and accurate detection of trace mycotoxins in agricultural products and food is significant for ensuring food safety and public health. Herein, a deep learning-assisted and entropy-driven catalysis (EDC)-Argonaute powered fluorescence single-p...