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Chromatography, High Pressure Liquid

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Benzoyl Chloride Derivatization Coupled With Liquid Chromatography-Mass Spectrometry for the Simultaneous Quantification of Molnupiravir and Its Metabolite β-d-N-hydroxycytidine in Human Plasma.

Journal of separation science
A sensitive and efficient method for simultaneous quantifying molnupiravir and its active metabolite β-d-N-hydroxycytidine in human plasma was developed by combining chemical derivatization with liquid chromatography-tandem mass spectrometry. Through...

Analytical Quality by Design for Chiral Pharmaceuticals: A Robust HPLC Method for Upadacitinib Enantiomeric Quantification.

Chirality
Ensuring the enantiomeric purity of chiral pharmaceuticals is paramount for patient safety and therapeutic efficacy. Upadacitinib (UPA), a vital Janus kinase 1 (JAK-1) inhibitor for rheumatoid arthritis treatment, exemplifies this need. This study re...

Optimization of polyphenols extraction by deep eutectic solvent from broccoli stem and characterization of their composition and antioxidative effects.

Scientific reports
Broccoli stem is known to possess abundant polyphenols. To efficiently extract polyphenols from broccoli stems, we herein describe an updated deep eutectic solvent extraction (DESE) method. Response surface modeling was utilized for optimization of t...

Identification of potential markers of elevated anticandidal activity of propolis extracts.

Journal of ethnopharmacology
ETHNOPHARMACOLOGICAL RELEVANCE: For centuries, propolis has been one of the most important and popular antimicrobial (antibacterial and antifungal) agents used in traditional medicine worldwide, including Central and Eastern Europe. Despite centuries...

Prediction of Chromatographic Retention Time of a Small Molecule from SMILES Representation Using a Hybrid Transformer-LSTM Model.

Journal of chemical information and modeling
Accurate retention time (RT) prediction in liquid chromatography remains a significant consideration in molecular analysis. In this study, we explore the use of a transformer-based language model to predict RTs by treating simplified molecular input ...

Machine learning for predicting retention times of chiral analytes chromatographically separated by CMPA technique.

Journal of chromatography. A
Chiral mobile phase additive (CMPA) technique is an attractive method for chromatographic enantioseparation of chiral analytes. However, establishing chromatographic separation and analysis methods for given chiral analytes often requires extensive t...

Precise classification of traditional Chinese medicine sources using intelligent fusion of hyperspectral imaging-mass spectrometry data combined with machine learning: A case study of American ginseng.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
The application of artificial intelligence in traditional Chinese medicine (TCM) has become a hot topic in the scientific community. American ginseng (AG), a perennial herb with a rich history, is widely utilized in clinical settings due to its diver...

Machine learning-driven insights into retention mechanism in IAM chromatography of anticancer sulfonamides: Implications for biological efficacy.

Journal of chromatography. A
Machine learning (ML) tools offer new opportunities in drug discovery, especially for enhancing our understanding of molecular interactions with biological systems. This study develops a comprehensive quantitative structure-retention relationship (QS...

Comprehensive quality evaluation of crude material of Ligusticum chuanxiong Hort. through high performance liquid chromatography coupled with DenseNet-121 assisted hyperspectral imaging and anti-thrombotic zebrafish bioassay.

Journal of pharmaceutical and biomedical analysis
An innovative, integrated strategy was developed for rapid and comprehensive quality assessment of Ligusticum chuanxiong Hort., the key raw material for Guanxinning tablets. This approach simultaneously evaluates both chemical composition and biologi...

Metabolomics and machine learning identify urine metabolic characteristics and potential biomarkers for severe Mycoplasma pneumoniae pneumonia.

Scientific reports
To study the differences in the urine metabolome between pediatric patients with severe Mycoplasma pneumoniae pneumonia (SMPP) and those with general Mycoplasma pneumoniae pneumonia (GMPP) via non-targeted metabolomics method, and potential biomarker...