Adenosine Triphosphate Protects from Elevated Extracellular Calcium-Induced Damage in Human Proximal Kidney Cells: Using Deep Learning to Predict Cytotoxicity.

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
PMID: 36126285

Abstract

BACKGROUND/AIMS: In kidney, extracellular [Ca] can modulate intracellular [Ca] to control key cellular processes. Hence, extracellular [Ca] is normally maintained within narrow range. We tested effect of extracellular ATP on viability of human proximal (HK-2) cells at high calcium. Modulation of intracellular calcium was assessed by imaging cytosolic [Ca], and expression of calcium-binding proteins (CaBPs). We present an artificial intelligence enabled deep learning model for prediction of injury and protection against extracellular [Ca] in HK-2 cells.

Authors

  • Rawad Hodeify
    Department of Biotechnology, School of Arts and Sciences, American University of Ras Al Khaimah, Ras Al Khaimah, United Arab Emirates, rawad.hodeify@aurak.ac.ae.
  • Arfan Ghani
    Department of Computer Science and Engineering, School of Engineering, American University of Ras al Khaimah, Ras al Khaimah, United Arab Emirates.
  • Rachel Matar
    Department of Biotechnology, School of Arts and Sciences, American University of Ras Al Khaimah, Ras Al Khaimah, United Arab Emirates.
  • Cijo George Vazhappilly
    Department of Biotechnology, School of Arts and Sciences, American University of Ras Al Khaimah, Ras Al Khaimah, United Arab Emirates.
  • Maxime Merheb
    Department of Biotechnology, School of Arts and Sciences, American University of Ras Al Khaimah, Ras Al Khaimah, United Arab Emirates.
  • Hussain Al Zouabi
    Department of Biotechnology, School of Arts and Sciences, American University of Ras Al Khaimah, Ras Al Khaimah, United Arab Emirates.
  • John Marton
    Department of Biotechnology, School of Arts and Sciences, American University of Ras Al Khaimah, Ras Al Khaimah, United Arab Emirates.

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