[Preparation of monoclonal antibody against cystatin C and establishment of its immunoassay system].

Journal: Wei sheng yan jiu = Journal of hygiene research
PMID:

Abstract

OBJECTIVE: To prepare human cystatin C( CysC) recombinant protein and produce monoclonal antibodies with high affinity and specificity. Develop a competitive ELISA detection system to detect of CysC in human serum.

Authors

  • Yizhan Yu
    School of Life Sciences, Nanjing University, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing 210000, China.
  • Tingting Yang
    School of Life Sciences, Nanjing University, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing 210000, China.
  • Pei Liu
    School of Life Sciences, Nanjing University, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing 210000, China.
  • Nianchao Wang
    School of Life Sciences, Nanjing University, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing 210000, China.
  • Yan Lu
    National Institute of Standards and Technology.
  • Yahong Huang
    School of Life Sciences, Nanjing University, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing 210000, China.
  • Pingping Shen
    School of Life Sciences, Nanjing University, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing 210000, China.