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Immunoassay

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Tyramide signal amplification for a highly sensitive multiplex immunoassay based on encoded hydrogel microparticles.

The Analyst
Proteins play a crucial role as mediators of immune regulation, homeostasis, and metabolism, making their quantification essential for understanding disease mechanisms in biomedical research and clinical diagnostics. However, conventional methods whe...

Dual-Mode Fluorescent/Intelligent Lateral Flow Immunoassay Based on Machine Learning Algorithm for Ultrasensitive Analysis of Chloroacetamide Herbicides.

Analytical chemistry
Given the harmful effect of pesticide residues, it is essential to develop portable and accurate biosensors for the analysis of pesticides in agricultural products. In this paper, we demonstrated a dual-mode fluorescent/intelligent (DM-f/DM-i) latera...

LSPR-susceptible metasurface platform for spectrometer-less and AI-empowered diagnostic biomolecule detection.

Analytica chimica acta
In response to the growing demand for biomolecular diagnostics, metasurface (MS) platforms based on high-Q resonators have demonstrated their capability to detect analytes with smart data processing and image analysis technologies. However, high-Q re...

Machine learning powered detection of biological toxins in association with confined lateral flow immunoassay (c-LFA).

The Analyst
Biological weapons, primarily dispersed as aerosols, can spread not only to the targeted area but also to adjacent regions following the movement of air driven by wind. Thus, there is a growing demand for toxin analysis because biological weapons are...

Ultrasensitive Detection of Blood-Based Alzheimer's Disease Biomarkers: A Comprehensive SERS-Immunoassay Platform Enhanced by Machine Learning.

ACS chemical neuroscience
Accurate and early disease detection is crucial for improving patient care, but traditional diagnostic methods often fail to identify diseases in their early stages, leading to delayed treatment outcomes. Early diagnosis using blood derivatives as a ...

A machine-learning-integrated portable electrochemiluminescence sensing platform for the visualization and high-throughput immunoassays.

Talanta
Electrochemiluminescence (ECL)-based point-of-care testing (POCT) has the potential to facilitate the rapid identification of diseases, offering advantages such as high sensitivity, strong selectivity, and minimal background interference. However, as...

Deep-Learning-Assisted Digital Fluorescence Immunoassay on Magnetic Beads for Ultrasensitive Determination of Protein Biomarkers.

Analytical chemistry
Digital fluorescence immunoassay (DFI) based on random dispersion magnetic beads (MBs) is one of the powerful methods for ultrasensitive determination of protein biomarkers. However, in the DFI, improving the limit of detection (LOD) is challenging s...

Using Machine Learning and Optical Microscopy Image Analysis of Immunosensors Made on Plasmonic Substrates: Application to Detect the SARS-CoV-2 Virus.

ACS sensors
In this article, we introduce a diagnostic platform comprising an optical microscopy image analysis system coupled with machine learning. Its efficacy is demonstrated in detecting SARS-CoV-2 virus particles at concentrations as low as 1 PFU (plaque-f...

Dual-Mode Colorimetric/SERS Lateral Flow Immunoassay with Machine Learning-Driven Optimization for Ultrasensitive Mycotoxin Detection.

Analytical chemistry
Detecting and quantifying mycotoxins using LFIA are challenging due to the need for high sensitivity and accuracy. To address this, a dual-mode colorimetric-SERS LFIA was developed for detecting deoxynivalenol (DON). Rhodium nanocores provided strong...

Deep-Learning-Assisted Microfluidic Immunoassay via Smartphone-Based Imaging Transcoding System for On-Site and Multiplexed Biosensing.

Nano letters
Point-of-care testing (POCT) with multiplexed capability, ultrahigh sensitivity, affordable smart devices, and user-friendly operation is critically needed for clinical diagnostics and food safety. This study presents a deep-learning-assisted microfl...