Glycoside Hydrolases

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Optimizing agarase production from sp. using response surface methodology and machine learning models.

Environmental technology Apr 5, 2025
Agarase enzymes are critical in industries like food, cosmetics, and medicine where they play a critical role in DNA recovery, food gelling, cosmetic formulations, and waste treatment. However, current agarase sources often face limitations related t...
Machine Learning Temperature Hydrogen-Ion Concentration Glycoside Hydrolases Agar Alteromonadaceae
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Optimization of α-L-arabinofuranosidase CcABF on clarification and beneficial active substances in fermented ginkgo kernel juice by artificial neural network and genetic algorithm.

Food chemistry Apr 15, 2024
This study aimed at using α-L-arabinofuranosidase CcABF to improve the clarity and active substances in fermented ginkgo kernel juice by artificial neural network (ANN) modeling and genetic algorithm (GA) optimization. A credible three-layer feedforw...
Neural Networks, Computer Algorithms Antioxidants Fermentation Fruit and Vegetable Juices Food Handling Glycoside Hydrolases Ginkgo biloba
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Machine learning reveals sequence-function relationships in family 7 glycoside hydrolases.

The Journal of biological chemistry Jul 1, 2021
Family 7 glycoside hydrolases (GH7) are among the principal enzymes for cellulose degradation in nature and industrially. These enzymes are often bimodular, including a catalytic domain and carbohydrate-binding module (CBM) attached via a flexible li...
Machine Learning Catalytic Domain Glycoside Hydrolases
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Enhanced Fermentable Sugar Production from Enteromorpha Polysaccharides by the Crude Enzymes of Vibrio sp. H11.

Journal of molecular microbiology and biotechnology Mar 6, 2020
In recent years, large-scale outbreaks of the green alga Enteromorpha prolifera in China's offshore waters have posed a serious threat. This study aimed to improve Enteromorpha polysaccharide (EP) enzymatic sugar production using the hydrolase system...
Bacterial Proteins Polysaccharides Ulva Hydrolysis Fermentation Biofuels Glycoside Hydrolases Industrial Microbiology Vibrio
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Development and characterization of an α-l-rhamnosidase mutant with improved thermostability and a higher efficiency for debittering orange juice.

Food chemistry Nov 16, 2017
The glycoside hydrolase, α-l-rhamnosidase, could remove the bitter taste of naringin from citrus juices. However, most α-l-rhamnosidases are easily deactivated at high temperatures, limiting the practice in debittering citrus juices. The V529A mutant...
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Temperature Fruit and Vegetable Juices Taste Aspergillus niger Glycoside Hydrolases Citrus sinensis Directed Molecular Evolution Mutagenesis, Site-Directed Protein Engineering
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Large-scale genomic survey with deep learning-based method reveals strain-level phage specificity determinants.

GigaScience Jan 2, 2024
BACKGROUND: Phage therapy, reemerging as a promising approach to counter antimicrobial-resistant infections, relies on a comprehensive understanding of the specificity of individual phages. Yet the significant diversity within phage populations prese...
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Deep Learning Genomics Bacteria Glycoside Hydrolases Genome, Viral Bacteriophages Genome, Bacterial Viral Tail Proteins Host Specificity
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