AIMC Topic: Steroid Hydroxylases

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Determination of total plasma oxysterols by enzymatic hydrolysis, solid phase extraction and liquid chromatography coupled to mass-spectrometry.

Journal of pharmaceutical and biomedical analysis Dec 21, 2017
The potential use of cholesterol esterases was tested to avoid alkaline hydrolysis for cleavage of plasma esterified oxysterols. The enzymatic hydrolysis was optimized by testing two sources of enzyme-Pseudomonas and bovine pancreas, presence of surf...
Humans Reproducibility of Results Animals Male Chromatography, Liquid Bacterial Proteins Tandem Mass Spectrometry Pseudomonas aeruginosa Solid Phase Extraction Hydrolysis Animals, Genetically Modified Calibration Reference Standards Cytochrome P450 Family 7 Oxysterols Rats, Inbred F344 Steroid Hydroxylases Sterol Esterase
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