AIMC Topic: Templates, Genetic

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Prediction of PCR amplification from primer and template sequences using recurrent neural network.

Scientific reports Apr 5, 2021
We have developed a novel method to predict the success of PCR amplification for a specific primer set and DNA template based on the relationship between the primer sequence and the template. To perform the prediction using a recurrent neural network...
Neural Networks, Computer Algorithms Reproducibility of Results DNA Primers Computational Biology Base Sequence Polymerase Chain Reaction Templates, Genetic
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Predictable and precise template-free CRISPR editing of pathogenic variants.

Nature Nov 7, 2018
Following Cas9 cleavage, DNA repair without a donor template is generally considered stochastic, heterogeneous and impractical beyond gene disruption. Here, we show that template-free Cas9 editing is predictable and capable of precise repair to a pre...
Humans Machine Learning Reproducibility of Results Fibroblasts CRISPR-Cas Systems Alleles HCT116 Cells HEK293 Cells Base Sequence Gene Editing K562 Cells Substrate Specificity DNA Repair CRISPR-Associated Protein 9 Hermanski-Pudlak Syndrome Menkes Kinky Hair Syndrome Templates, Genetic
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