The ability to detect nucleic acids at ever-lower detection limits is crucial in molecular science, enabling disease management to shift from reactive response to proactive prediction and personalized medicine. This review synthesizes the technologic...
Multi-template polymerase chain reaction (PCR) is a critical technique enabling the parallel amplification of diverse DNA molecules, thereby facilitating applications in fields from quantitative molecular biology to DNA data storage. However, non-hom...
The introduction of PCR into forensic science and the rapid increases in the sensitivity, specificity and discrimination power of DNA profiling that followed have been fundamental in shaping the field of forensic biology. Despite these developments, ...
The use of PCR is widespread in biological fields. Some fields, such as forensic biology, push PCR to its limits as DNA profiling may be required in short timeframes, may be produced from minute amounts of starting material, and may be required to pe...
BACKGROUND: Pneumocystis jirovecii pneumonia (PJP) is an interstitial pneumonia caused by pneumocystis jirovecii (PJ). The diagnosis of PJP primarily relies on the detection of the pathogen from lower respiratory tract specimens. However, it faces ch...
Innovations in molecular diagnostics have often evolved through the study of hematologic malignancies. Examples include the pioneering characterization of the Philadelphia chromosome by cytogenetics in the 1970s, the implementation of polymerase chai...
The International journal of biological markers
Dec 21, 2023
BACKGROUND: Cancer screening and early detection greatly increase the chances of successful treatment. However, most cancer types lack effective early screening biomarkers. In recent years, natural language processing (NLP)-based text-mining methods ...
Absolute quantification of biological samples provides precise numerical expression levels, enhancing accuracy, and performance for rare templates. Current methodologies, however, face challenges-flow cytometers are costly and complex, whereas fluore...
Plant pathogens cause severe losses to agricultural yield worldwide. Tracking plant health and early disease detection is important to reduce the disease spread and thus economic loss. Though visual scouting has been practiced from former times, dete...
Droplet-based dPCR offers many advantages over chip-based dPCR, such as lower processing cost, higher droplet density, higher throughput, while requiring less sample. However, the stochastic nature of droplet locations, uneven illuminations, and uncl...
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