AIMC Topic: HEK293 Cells

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Predicting the effect of CRISPR-Cas9-based epigenome editing.

eLife
Epigenetic regulation orchestrates mammalian transcription, but functional links between them remain elusive. To tackle this problem, we use epigenomic and transcriptomic data from 13 ENCODE cell types to train machine learning models to predict gene...

Silencing of optogenetic and chemogenetic transgenes in human iPSCs involves promoter methylation and methylation-independent mechanisms.

Epigenetics
The transplantation of neural progenitor cells derived from induced pluripotent stem cells (iPSCs) has therapeutic potential for the treatment of neurological diseases. However, the functional integration of transplanted iPSC-derived neurons into hos...

Polymer-Functionalized Liposomes as Universal Nanocarriers for Drug Delivery: Single Particle Insights on Size-Dependent Performance and Intracellular Behavior.

ACS applied materials & interfaces
Nanomedicine requires smart delivery systems that are precise, robust, and universal. While liposomes are established vehicles in drug delivery, their full potential is challenged by limited stability, leakage, insufficient response, and limited insi...

Proteome-wide computational analyses reveal links between protein condensate formation and RNA biology.

Science advances
Biomolecular condensates mediate dynamic compartmentalization of cellular processes. The multivalent interactions that underlie biomolecular condensation are often promoted by intrinsically disordered regions (IDRs) within proteins. Although the role...

4-Hydroxy-2,5-dihydrothiazole derivatives as a new class of small-molecule antibiotics for MRSA: AI-integrated design, chemical synthesis and biological evaluation.

European journal of medicinal chemistry
Staphylococcus aureus (S. aureus) is one of the most concerned Gram-positive bacteria due to its resistance to the commonly used antibiotics, methicillin. To address the threat of methicillin-resistant S. aureus (MRSA), new classes of antibiotics are...

Developing a predictive QSAR model for FGFR-1 inhibitors: integrating computational and experimental validation.

Journal of computer-aided molecular design
The traditional drug discovery process is often lengthy, costly, and characterized by a high failure rate. There is a pressing need for innovative strategies to optimize this process and improve the chances of identifying effective therapeutic candid...

Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids.

Nature communications
Understanding SARS-CoV-2 human protein-protein interactions (PPIs) and the host response to infection is essential for developing effective COVID-19 antivirals. However, how the ancestral virus and its variants remodel virus-host protein assemblies i...

Artificial intelligence-driven discovery of novel scaffolds for selective TLR7 antagonists and their application in enhancing mRNA translation efficiency.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
Toll-like receptor 7 (TLR7) is crucial in the innate immune response, responsible for recognizing single-stranded RNA from external pathogens and initiating the production of inflammatory cytokines and type I interferons. Despite the potential therap...

Substrate Specificity of the Organic Cation Transporters MATE1 and MATE2K and Functional Overlap with OCT1 and OCT2.

Journal of medicinal chemistry
The multidrug and toxin extrusion proteins MATE1 and MATE2K may determine the pharmacokinetics and drug-drug interactions of many drugs. However, their substrate spectrum and synergy with organic cation transporters OCT1 and OCT2 remain incompletely ...

Stem loop binding protein promotes SARS-CoV-2 replication via -1 programmed ribosomal frameshifting.

Signal transduction and targeted therapy
The -1 programmed ribosomal frameshifting (-1 PRF) in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial for keeping the balance between pp1a and pp1ab polyproteins. To date, the host factors influencing this process remain poorl...